Evidence map›Paper›PMID 28862339›Full record

ArticleCurrent protocols in cell biology2017

Determination of Membrane Protein Distribution on the Nuclear Envelope by Single-Point Single-Molecule FRAP.

Krishna C Mudumbi, Weidong Yang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols in cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Krishna C MudumbiDepartment of Biology, Temple University, Philadelphia, Pennsylvania.
Weidong YangDepartment of Biology, Temple University, Philadelphia, Pennsylvania.
Temple University · US

Funding

Super-resolution Microscopy Study of Molecular Transport MechanismsR35GM122552 · NIGMS · TEMPLE UNIV OF THE COMMONWEALTH · PI Weidong Yang · 2017 to 2026
$4.5M
Structure and Function of the Ciliary Pore ComplexR01GM116204 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI VERHEY, KRISTEN J., YANG, WEIDONG · 2015 to 2018
$2.1M
Three-Dimensional Super-Resolution Microscopy Study of Nuclear TransportR01GM097037 · NIGMS · TEMPLE UNIV OF THE COMMONWEALTH · PI YANG, WEIDONG · 2011 to 2015
$1.3M
Single-Molecule Three-Dimensional Snapshots of Nuclear TransportR15GM094041 · NIGMS · BOWLING GREEN STATE UNIVERSITY · PI YANG, WEIDONG · 2010 to 2010
$348k
NIGMS NIH HHS R01 GM097037NIGMS NIH HHS R01 GM116204NIGMS NIH HHS R15 GM094041NIGMS NIH HHS R35 GM122552
6 · The paper itself

Abstract

Nuclear envelope transmembrane proteins (NETs) are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM) in eukaryotic cells. The abnormal distribution of NETs has been associated with many human diseases. However, quantitative determination of the spatial distribution and translocation dynamics of NETs on the ONM and INM is still very limited in currently existing approaches. Here we demonstrate a single-point single-molecule fluorescence recovery after photobleaching (FRAP) microscopy technique that enables quick determination of distribution and translocation rates for NETs in vivo. © 2017 by John Wiley & Sons, Inc.

Indexed as

DiffusionFluorescence Recovery After PhotobleachingHeLa CellsHumansMembrane ProteinsMicroscopy, ConfocalNuclear EnvelopeTissue Culture TechniquesMembrane ProteinsCellular imagingFRAPmembrane biophysicssuper-resolutiontransmembrane protein

Identifiers

PMID28862339
PMCPMC5879780
OpenAlexW2751444528

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.