Evidence map›Paper›PMID 28861790›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Methods of Protein Misfolding Cyclic Amplification.

Natallia Makarava, Regina Savtchenko, Ilia V Baskakov

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Highly sensitive chemiluminescence imaging of misfolded proteins in neurodegenerative models.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Yeast Prions: Discovery, Nature, Cellular Manipulation and Implication.Journal of microbiology and biotechnology · 2025
    Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Natallia MakaravaDepartment of Anatomy and Neurobiology, Center for Biomedical Engineering and Technology, University of Maryland School of Medicine, 111 Penn St., Baltimore, MD, 21201, USA.
Regina SavtchenkoDepartment of Anatomy and Neurobiology, Center for Biomedical Engineering and Technology, University of Maryland School of Medicine, 111 Penn St., Baltimore, MD, 21201, USA.
Ilia V BaskakovDepartment of Anatomy and Neurobiology, Center for Biomedical Engineering and Technology, University of Maryland School of Medicine, 111 Penn St., Baltimore, MD, 21201, USA. baskakov@umaryland.edu.

Funding

Self-Propagating Mechanism of Prion DiseasesR01NS045585 · NINDS · UNIVERSITY OF MD BIOTECHNOLOGY INSTITUTE · PI Ilia V Baskakov · 2003 to 2026
$9.6M
Elucidating molecular structure of mammalian prionsR01NS074998 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 2012 to 2015
$1.4M
Elucidating molecular structure of mammalian prionsR56NS074998 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 2011 to 2011
$405k
NINDS NIH HHS R01 NS045585NINDS NIH HHS R01 NS074998NINDS NIH HHS R56 NS074998
6 · The paper itself

Abstract

Protein misfolding cyclic amplification (PMCA) amplifies infectious prions in vitro. Over the past decade, PMCA has become an essential tool in prion research. The current chapter describes in detail the PMCA format with beads (PMCAb) and several methods that rely on PMCAb for assessing strain-specific prion amplification rates, for selective amplification of subtypes of PrP

Indexed as

Biological AssayAnimalsBrainBrain ChemistryCricetulusEndopeptidase KGene ExpressionHumansMicrospheresProtein FoldingPrPC ProteinsPrPSc ProteinsRibonuclease, PancreaticRNAScrapieSonicationEndopeptidase KPrPC ProteinsPrPSc ProteinsRibonuclease, PancreaticRNAPrion detectionPrion diseasesPrion proteinPrion strainsProtein misfolding cyclic amplificationReplication rateSelf-propagating protein statesSonication

Identifiers

PMID28861790
PMCPMC7052956

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.