ArticleCurrent microbiology2018
Extracellular Expression in Aspergillus niger of an Antibody Fused to Leishmania sp. Antigens.
Article in Current microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Establishment of the Genetic Transformation System ofJournal of fungi (Basel, Switzerland) · 2026Article
- Heterochromatin Protein Activates the Amylase Expression Pathway and Its Application to Recombinant Protein Expression in Penicillium oxalicum.Current microbiology · 2025Article
- Cloning, recombinant expression, purification, and functional characterization of AGAAN antibacterial peptide.3 Biotech · 2023Article
- Effect of Shock Waves on the Growth ofJournal of fungi (Basel, Switzerland) · 2022Article
- Mammalian Cell Culture as a Platform for Veterinary Vaccines.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Recombinant Protein Production and Purification of Insoluble Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Constructing a novel expression system by specific activation of amylase expression pathway in Penicillium.Microbial cell factories · 2020Article
- Improved Homologous Expression of the Acidic Lipase fromJournal of microbiology and biotechnology · 2020Article
- A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals.BioMed research international · 2019Review
- Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review.Frontiers in pharmacology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleoside hydrolase and sterol 24-c-methyltransferase, two antigenic proteins of Leishmania sp., were expressed in Aspergillus niger. Genetic transformation of conidia was achieved using underwater shock waves. scFv antibody addressed to DEC205, a receptor of dendritic cells, was fused to two proteins of Leishmania sp. Receptor 205 has a relevant role in the immune system in mammals; it can modulate T cell response to different antigens. Extracellular expression strategy of recombinant antibody was achieved using a fragment of native glucoamylase A (514 aa) as a carrier. Fermentations in shake flasks showed that the recombinant protein (104 kDa) was expressed and secreted only when maltose was used as carbon source; on the contrary, the expression was highly repressed in presence of xylose. Noteworthy, recombinant protein was secreted without glucoamylase-carrier and accumulation at intracellular level was not observed. The results presented here demonstrate the high value of Aspergillus niger as biotechnological platform for recombinant antibodies against Leishmania sp. at low cost. To the best of our knowledge, this is the first report about the recombinant expression of antigenic proteins of Leishmania sp. in filamentous fungi. The protein obtained can be used to explore novel strategies to induce immunity against Leishmania sp. or it can be employed in diagnostic kits to detect this neglected disease.
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