Evidence map›Paper›PMID 28861128›Full record

ArticleClinical epigenetics2017

Pyrosequencing quantified methylation level of miR-124 predicts shorter survival for patients with myelodysplastic syndrome.

Hong Wang, Tong-Tong Zhang, Song Jin, Hong Liu, Xiang Zhang, Chang-Geng Ruan, De-Pei Wu, Yue Han, Xiao-Qin Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical epigenetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
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  4. Epigenetic regulation ofMolecular therapy. Nucleic acids · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Hong Wang *Jiangsu Institute of Haematology, Institute of Blood and Marrow Transplantation, Department of Haematology, Collaborative Innovation of Haematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou, 215000 China.
Tong-Tong Zhang *Jiangsu Institute of Haematology, Institute of Blood and Marrow Transplantation, Department of Haematology, Collaborative Innovation of Haematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou, 215000 China.
Song Jin *Jiangsu Institute of Haematology, Institute of Blood and Marrow Transplantation, Department of Haematology, Collaborative Innovation of Haematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou, 215000 China.
Hong LiuJiangsu Institute of Haematology, Institute of Blood and Marrow Transplantation, Department of Haematology, Collaborative Innovation of Haematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou, 215000 China.
Xiang ZhangJiangsu Institute of Haematology, Institute of Blood and Marrow Transplantation, Department of Haematology, Collaborative Innovation of Haematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou, 215000 China.
Chang-Geng RuanJiangsu Institute of Haematology, Institute of Blood and Marrow Transplantation, Department of Haematology, Collaborative Innovation of Haematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou, 215000 China.
De-Pei WuJiangsu Institute of Haematology, Institute of Blood and Marrow Transplantation, Department of Haematology, Collaborative Innovation of Haematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou, 215000 China.
Yue HanJiangsu Institute of Haematology, Institute of Blood and Marrow Transplantation, Department of Haematology, Collaborative Innovation of Haematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, 188 Shi Zi Street, Suzhou, 215000 China.
Xiao-Qin WangDepartment of Haematology, Huashan Hospital of Fudan University, 12 Wulumuqi Road Central, Shanghai, 200040 China.
First Affiliated Hospital of Soochow University · CNSoochow University · CNFudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAberrant CpG island methylation has been increasingly recognized as a common event in myelodysplastic syndrome (MDS). To date, most of the previous studies of miR-124 in MDS have focused on epigenetic changes and little is known about the underlying mechanism through which miR-124 regulates

resultsIn the present study, we employed pyrosequencing analysis to quantify the methylation levels of upstream regions of the miR-124 genes (miR-124-1, miR-124-2 and miR-124-3) in 56 primary MDS patients. We found the three miR-124 genes were methylated in MDS patients. Univariate analysis revealed that the World Health Organization (WHO) classification, marrow blast count, karyotype, International Prognostic Scoring System (IPSS), mean corpuscular volume, as well as high methylation of miR-124-1, miR-124-2 and miR-124-3 were significantly related to overall survival. In leukaemia-free survival, patients who were older and had an advanced WHO classification, high marrow blast counts, high IPSS risk and high methylation of miR-124-1 and miR-124-2 progressed rapidly to acute myeloid leukaemia. Multivariate analysis demonstrated that high methylation of miR-124-3 was an independent factor of overall survival. Median survival of patients with high miR-124-3 methylation was significantly shorter (7.6 months) than patients with low methylation (32.7 months;

conclusionsHigh methylation of miR-124-3 predicts shorter survival for patients with MDS, which may be a useful prognostic marker in MDS.

Indexed as

DNA MethylationAdultAgedAged, 80 and overCell Line, TumorCpG IslandsCyclin-Dependent Kinase 6FemaleHumansMaleMicroRNAsMiddle AgedMyelodysplastic SyndromesPrognosisPromoter Regions, GeneticSequence Analysis, DNACDK6 protein, humanCyclin-Dependent Kinase 6MicroRNAsMIRN124 microRNA, humanMDSMethylationmiR-124PrognosisPyrosequencing

Identifiers

PMID28861128
PMCPMC5577794
OpenAlexW2751264682

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.