Evidence map›Paper›PMID 28860476›Full record

ArticleScientific reports2017

Quantifying perinatal transmission of Hepatitis B viral quasispecies by tag linkage deep sequencing.

Yushen Du, Xiumei Chi, Chong Wang, Jing Jiang, Fei Kong, Hongqing Yan, Xiaomei Wang, Jie Li, Nicholas C Wu, Lei Dai and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Yushen DuDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Xiumei ChiHepatology, The 1st hospital of Jilin University, Changchun, 130031, China.
Chong WangHepatology, The 1st hospital of Jilin University, Changchun, 130031, China.
Jing JiangEpidemiology, The 1st hospital of Jilin University, Changchun, 130031, China.
Fei KongHepatology, The 1st hospital of Jilin University, Changchun, 130031, China.
Hongqing YanHepatology, The 1st hospital of Jilin University, Changchun, 130031, China.
Xiaomei WangHepatology, The 1st hospital of Jilin University, Changchun, 130031, China.
Jie LiDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China.
Nicholas C WuDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Lei DaiDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Tian-Hao ZhangDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Sara ShuDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Jian ZhouDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
Janice M YoshizawaDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
Xinmin LiDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
Debika BhattacharyaDepartment of Medicine, Division of Infectious Diseases, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
Ting-Ting WuDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA.
Junqi NiuHepatology, The 1st hospital of Jilin University, Changchun, 130031, China. junqiniu@aliyun.com.
Ren SunDepartment of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA. rsun@mednet.ucla.edu.

Funding

Virology CoreP01CA177322 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SUN, REN · 2014 to 2018
$8.7M
Lamivudine and its Impact on Perinatal HBV Transmission in HIV/HBV CoinfectionR01AI100748 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BHATTACHARYA, DEBIKA · 2012 to 2016
$1.9M
NCI NIH HHS P01 CA177322NIAID NIH HHS R01 AI100748
6 · The paper itself

Abstract

Despite full immunoprophylaxis, mother-to-child transmission (MTCT) of Hepatitis B Virus still occurs in approximately 2-5% of HBsAg positive mothers. Little is known about the bottleneck of HBV transmission and the evolution of viral quasispecies in the context of MTCT. Here we adopted a newly developed tag linkage deep sequencing method and analyzed the quasispecies of four MTCT pairs that broke through immunoprophylaxis. By assigning unique tags to individual viral sequences, we accurately reconstructed HBV haplotypes in a region of 836 bp, which contains the major immune epitopes and drug resistance mutations. The detection limit of minor viral haplotypes reached 0.1% for individual patient sample. Dominance of "a determinant" polymorphisms were observed in two children, which pre-existed as minor quasispecies in maternal samples. In all four pairs of MTCT samples, we consistently observed a significant overlap of viral haplotypes shared between mother and child. We also demonstrate that the data can be potentially useful to estimate the bottleneck effect during HBV MTCT, which provides information to optimize treatment for reducing the frequency of MTCT.

Indexed as

ChildDNA, ViralEvolution, MolecularFemaleHaplotypesHepatitis BHepatitis B virusHigh-Throughput Nucleotide SequencingHumansInfectious Disease Transmission, VerticalPhylogenyPregnancyPregnancy Complications, InfectiousQuasispeciesSequence Analysis, DNADNA, Viral

Identifiers

PMID28860476
PMCPMC5578979

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.