ArticlePLoS pathogens2017
The replication initiator protein of a geminivirus interacts with host monoubiquitination machinery and stimulates transcription of the viral genome.
Article in PLoS pathogens, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 71 citations in OpenAlex.
- Chromatin remodeling by HIRA and FAS2 restricts geminivirus pathogenesis in plants.Virology journal · 2026Article
- A SUMO interacting motif in the replication initiator protein of tomato yellow leaf curl virus is required for viral replication.Journal of virology · 2025Article
- Histone ZmH2B regulates resistance to the Southern corn leaf blight pathogen Bipolaris maydis in maize.BMC plant biology · 2025Article
- Geminivirus diseases of legumes in India: current status and approaches for management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Correction: The replication initiator protein of a geminivirus interacts with host monoubiquitination machinery and stimulates transcription of the viral genome.PLoS pathogens · 2024Article
- H3.1K27me1 loss confers Arabidopsis resistance to Geminivirus by sequestering DNA repair proteins onto host genome.Nature communications · 2023Article
- Article
- Differential expression of genes during recovery of Nicotiana tabacum from tomato leaf curl Gujarat virus infection.Planta · 2023Article
- Article
- REPercussions: how geminiviruses recruit host factors for replication.Frontiers in microbiology · 2023Review
- Article
- Insights into the multifunctional roles of geminivirus-encoded proteins in pathogenesis.Archives of virology · 2022Review
- An Overview of Chili Leaf Curl Disease: Molecular Mechanisms, Impact, Challenges, and Disease Management Strategies in Indian Subcontinent.Frontiers in microbiology · 2022Review
- Plant responses to geminivirus infection: guardians of the plant immunity.Virology journal · 2021Review
- RNA-Seq Transcriptome Analysis Provides Candidate Genes for Resistance toFrontiers in plant science · 2021Article
- A cassava protoplast system for screening genes associated with the response to South African cassava mosaic virus.Virology journal · 2020Article
- Article
- Stability of Begomoviral pathogenicity determinant βC1 is modulated by mutually antagonistic SUMOylation and SIM interactions.BMC biology · 2020Article
- Geminivirus-Encoded Proteins: Not All Positional Homologs Are Made Equal.Frontiers in microbiology · 2020Article
- Identification of Tomato Proteins That Interact With Replication Initiator Protein (Rep) of the Geminivirus TYLCV.Frontiers in plant science · 2020Article
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Geminiviruses constitute a group of plant viruses, with a ssDNA genome, whose replication in the nucleus of an infected cell requires the function of geminivirus-encoded replication initiator protein (Rep). Our results suggest that monoubiquitinated histone 2B (H2B-ub) promotes tri-methylation of histone 3 at lysine 4 (H3-K4me3) on the promoter of Chilli leaf curl virus (ChiLCV). We isolated homologues of two major components of the monoubiquitination machinery: UBIQUITIN-CONJUGATING ENZYME2 (NbUBC2) and HISTONE MONOUBIQUITINATION1 (NbHUB1) from N. benthamiana. ChiLCV failed to cause disease in NbUBC2-, and NbHUB1-silenced plants, at the same time, H2B-ub and H3-K4me3 modifications were decreased, and the occupancy of RNA polymerase II on the viral promoter was reduced as well. In further investigations, Rep protein of ChiLCV was found to re-localize NbUBC2 from the cytoplasm to the nucleoplasm, like NbHUB1, the cognate partner of NbUBC2. Rep was observed to interact and co-localize with NbHUB1 and NbUBC2 in the nuclei of the infected cells. In summary, the current study reveals that the ChiLCV Rep protein binds the viral genome and interacts with NbUBC2 and NbHUB1 for the monoubiquitination of histone 2B that subsequently promotes trimethylation of histone 3 at lysine 4 on ChiLCV mini-chromosomes and enhances transcription of the viral genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.