Evidence map›Paper›PMID 28859130›Full record

ArticlePloS one2017

Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46.

Manasi Balachandran, Richard J Giannone, David A Bemis, Stephen A Kania

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. TheMolecules (Basel, Switzerland) · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Manasi BalachandranDepartment of Biomedical and Diagnostic Sciences, The University of Tennessee, Knoxville, Tennessee, United States of America.
Richard J GiannoneChemical Sciences Division, Mass Spectrometry and Laser Spectrometry, Oakridge National Laboratories, Oakridge, Tennessee, United States of America.
David A BemisDepartment of Biomedical and Diagnostic Sciences, The University of Tennessee, Knoxville, Tennessee, United States of America.
Stephen A KaniaDepartment of Biomedical and Diagnostic Sciences, The University of Tennessee, Knoxville, Tennessee, United States of America.ORCID http://orcid.org/0000-0002-4490-7347
University of Tennessee at Knoxville · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.

Indexed as

Gene Expression Regulation, BacterialAdhesins, BacterialAmino Acid MotifsAminoacyltransferasesBacterial ProteinsBinding SitesCarrier ProteinsCell WallCysteine EndopeptidasesMutationPromoter Regions, GeneticProtein BindingStaphylococcal Protein AStaphylococcus aureusTranscription, GeneticAdhesins, BacterialAminoacyltransferasesBacterial ProteinsCarrier ProteinsCysteine Endopeptidasesfibrinogen-binding protein A, Staphylococcus aureusfibronectin-binding proteins, bacterialsortase AStaphylococcal Protein A

Identifiers

PMID28859130
PMCPMC5578664
OpenAlexW2750790223

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.