ArticlePloS one2017
Regulation of myoblast differentiation by metabolic perturbations induced by metformin.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Fibro-Adipogenic Progenitor Cell Alterations in Skeletal Muscle: Pathological Dysfunction or Adaptive Reprogramming?International journal of molecular sciences · 2026Review
- Metformin enhances external urethral sphincter integrity and restores continence via AMPK activation in a rat model of stress urinary incontinence.Animal models and experimental medicine · 2026Article
- Transcriptome Sequencing Analysis of the Effects of Metformin on the Regeneration of PlanarianGenes · 2025Article
- Article
- RSPO3 is a novel contraction-inducible factor identified in an "in vitro exercise model" using primary human myotubes.Scientific reports · 2022Article
- Ostarine-Induced Myogenic Differentiation in C2C12, L6, and Rat Muscles.International journal of molecular sciences · 2022Article
- Systemic cell therapy for muscular dystrophies : The ultimate transplantable muscle progenitor cell and current challenges for clinical efficacy.Stem cell reviews and reports · 2021Review
- miR-378a-3p Participates in Metformin's Mechanism of Action on C2C12 Cells under Hyperglycemia.International journal of molecular sciences · 2021Article
- Extracellular serine and glycine are required for mouse and human skeletal muscle stem and progenitor cell function.Molecular metabolism · 2021Article
- Effect of metformin on stem cells: Molecular mechanism and clinical prospect.World journal of stem cells · 2020Review
- Functional skeletal muscle constructs from transdifferentiated human fibroblasts.Scientific reports · 2020Article
- β-guanidinopropionic acid and metformin differentially impact autophagy, mitochondria and cellular morphology in developing C2C12 muscle cells.Journal of muscle research and cell motility · 2020Article
- Active Compound ofMolecules (Basel, Switzerland) · 2020Article
- Metformin: Sentinel of the Epigenetic Landscapes That Underlie Cell Fate and Identity.Biomolecules · 2020Review
- Activating transcription factor 3 coordinates differentiation of cardiac and hematopoietic progenitors by regulating glucose metabolism.Science advances · 2020Article
- Mitochondrial Function in Muscle Stem Cell Fates.Frontiers in cell and developmental biology · 2020Review
- A Promising Future for Stem-Cell-Based Therapies in Muscular Dystrophies-In Vitro and In Vivo Treatments to Boost Cellular Engraftment.International journal of molecular sciences · 2019Review
- Peptide YY (PYY) Is Expressed in Human Skeletal Muscle Tissue and Expanding Human Muscle Progenitor Cells.Frontiers in physiology · 2019Article
- Metformin Delays Satellite Cell Activation and Maintains Quiescence.Stem cells international · 2019Article
- Identifying the murine mammary cell target of metformin exposure.Communications biology · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The metabolic perturbation caused by calorie restriction enhances muscle repair by playing a critical role in regulating satellite cell availability and activity in the muscles of young and old mice. To clarify the underlying mechanisms we asked whether myoblast replication and differentiation are affected by metformin, a calorie restriction-mimicking drug. C2C12, a mouse myoblast cell line, readily differentiate in vitro and fuse to form myotubes. However, when incubated with metformin, C2C12 slow their replication and do not differentiate. Interestingly, lower doses of metformin promote myogenic differentiation. We observe that metformin treatment modulates the expression of cyclins and cyclin inhibitors thereby inducing a cell cycle perturbation that causes a delay in the G2/M transition. The effect of metformin treatment is reversible since after drug withdrawal, myoblasts can re-enter the cell cycle and/or differentiate, depending on culture conditions. Myoblasts cultured under metformin treatment fail to up-regulate MyoD and p21cip1, a key step in cell cycle exit and terminal differentiation. Although the details of the molecular mechanisms underlying the effect of the drug on myoblasts still need to be clarified, we propose that metformin negatively affects myogenic differentiation by inhibiting irreversible exit from the cell cycle through reduction of MyoD and p21cip1 levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.