ArticleNeuron2017
Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology.
Article in Neuron, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 69 citations in OpenAlex.
- A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling.Microsystems & nanoengineering · 2026Article
- High-throughput multiplex voltage-clamp/current-clamp evaluation of acutely isolated neurons.Nature protocols · 2026Review
- Editorial: The diversity of the plasticitome.Frontiers in synaptic neuroscience · 2026Article
- Characterizing optogenetically mediated rebound effects in anaesthetized mouse primary visual cortex.The Journal of physiology · 2025Article
- Computer vision-guided rapid and precise automated cranial microsurgeries in mice.Science advances · 2025Article
- Principles of visual cortex excitatory microcircuit organization.Innovation (Cambridge (Mass.)) · 2025Article
- The evolution of patch-clamp electrophysiology: Robotic, multiplex, and dynamic.Molecular pharmacology · 2025Review
- ARViS: a bleed-free multi-site automated injection robot for accurate, fast, and dense delivery of virus to mouse and marmoset cerebral cortex.Nature communications · 2024Article
- Closed-loop experiments and brain machine interfaces with multiphoton microscopy.Neurophotonics · 2024Review
- High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.Genetics · 2024Article
- A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents.Journal of neuroscience methods · 2023Article
- High-throughput combined voltage-clamp/current-clamp analysis of freshly isolated neurons.Cell reports methods · 2023Article
- Daily two-photon neuronal population imaging with targeted single-cell electrophysiology and subcellular imaging in auditory cortex of behaving mice.Frontiers in cellular neuroscience · 2023Article
- Automated microscope-independent fluorescence-guided micropipette.Biomedical optics express · 2021Article
- Automated Intracellular Pharmacological Electrophysiology for Ligand-Gated Ionotropic Receptor and Pharmacology Screening.Molecular pharmacology · 2021Article
- Harnessing non-destructive 3D pathology.Nature biomedical engineering · 2021Review
- In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Automatic deep learning-driven label-free image-guided patch clamp system.Nature communications · 2021Article
- Grand Challenge at the Frontiers of Synaptic Neuroscience.Frontiers in synaptic neuroscience · 2021Article
- TbScience advances · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed "blind," meaning that throughput for sampling some genetically or morphologically defined cell types is unacceptably low. One solution to this problem is to use two-photon microscopy to target fluorescently labeled neurons. Combining this with robotic automation is difficult, however, as micropipette penetration induces tissue deformation, moving target cells from their initial location. Here we describe a platform for automated two-photon targeted patch-clamp recording, which solves this problem by making use of a closed loop visual servo algorithm. Our system keeps the target cell in focus while iteratively adjusting the pipette approach trajectory to compensate for tissue motion. We demonstrate platform validation with patch-clamp recordings from a variety of cells in the mouse neocortex and cerebellum.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.