ArticleNeuron2017
Closed-Loop Real-Time Imaging Enables Fully Automated Cell-Targeted Patch-Clamp Neural Recording In Vivo.
Article in Neuron, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling.Microsystems & nanoengineering · 2026Article
- Principles of visual cortex excitatory microcircuit organization.Innovation (Cambridge (Mass.)) · 2025Article
- Redefining Molecular Probes for Monitoring Subcellular Environment: A Perspective.Analytical chemistry · 2024Review
- ARViS: a bleed-free multi-site automated injection robot for accurate, fast, and dense delivery of virus to mouse and marmoset cerebral cortex.Nature communications · 2024Article
- Closed-loop experiments and brain machine interfaces with multiphoton microscopy.Neurophotonics · 2024Review
- High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.Genetics · 2024Article
- Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps.Sensors (Basel, Switzerland) · 2023Article
- A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents.Journal of neuroscience methods · 2023Article
- Daily two-photon neuronal population imaging with targeted single-cell electrophysiology and subcellular imaging in auditory cortex of behaving mice.Frontiers in cellular neuroscience · 2023Article
- Discovering sparse control strategies in neural activity.PLoS computational biology · 2022Article
- Multiscale, multi-perspective imaging assisted robotic microinjection of 3D biological structures.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2021Article
- Automated microscope-independent fluorescence-guided micropipette.Biomedical optics express · 2021Article
- Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts.Bio-protocol · 2021Article
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
- Harnessing non-destructive 3D pathology.Nature biomedical engineering · 2021Review
- In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Grand Challenge at the Frontiers of Synaptic Neuroscience.Frontiers in synaptic neuroscience · 2021Article
- Single neuron recording: progress towards high-throughput analysis.Bioelectronics in medicine · 2020Article
- Article
- Robotic platform for microinjection into single cells in brain tissue.EMBO reports · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
Abstract
Targeted patch-clamp recording is a powerful method for characterizing visually identified cells in intact neural circuits, but it requires skill to perform. We previously developed an algorithm that automates "blind" patching in vivo, but full automation of visually guided, targeted in vivo patching has not been demonstrated, with currently available approaches requiring human intervention to compensate for cell movement as a patch pipette approaches a targeted neuron. Here we present a closed-loop real-time imaging strategy that automatically compensates for cell movement by tracking cell position and adjusting pipette motion while approaching a target. We demonstrate our system's ability to adaptively patch, under continuous two-photon imaging and real-time analysis, fluorophore-expressing neurons of multiple types in the living mouse cortex, without human intervention, with yields comparable to skilled human experimenters. Our "imagepatching" robot is easy to implement and will help enable scalable characterization of identified cell types in intact neural circuits.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.