ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2017
Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Pigmentation biosynthesis influences the microbiome in sea urchins.Proceedings. Biological sciences · 2022Article
- Sperm lacking Bindin are infertile but are otherwise indistinguishable from wildtype sperm.Scientific reports · 2021Article
- Light-induced, spatiotemporal control of protein in the developing embryo of the sea urchin.Developmental biology · 2021Article
- Functional contribution of DCLKs in sea urchin development.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- CRISPR/Cas9 mutagenesis reveals a role for ABCB1 in gut immune responses to Vibrio diazotrophicus in sea urchin larvae.The Journal of experimental biology · 2021Article
- Precision genome editing using cytosine and adenine base editors in mammalian cells.Nature protocols · 2021Article
- Embryo, larval, and juvenile staging of Lytechinus pictus from fertilization through sexual maturation.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors.Nature biotechnology · 2020Review
- A tumor suppressor Retinoblastoma1 is essential for embryonic development in the sea urchin.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- The painted sea urchin, Lytechinus pictus, as a genetically-enabled developmental model.Methods in cell biology · 2019Review
- CRISPR/Cas9-mediated genome editing in sea urchins.Methods in cell biology · 2019Review
- Acceleration of cancer science with genome editing and related technologies.Cancer science · 2018Review
- Editing the human genome: where ART and science intersect.Journal of assisted reproduction and genetics · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundA single base pair mutation in the genome can result in many congenital disorders in humans. The recent gene editing approach using CRISPR/Cas9 has rapidly become a powerful tool to replicate or repair such mutations in the genome. These approaches rely on cleaving DNA, while presenting unexpected risks.
resultsIn this study, we demonstrate a modified CRISPR/Cas9 system fused to cytosine deaminase (Cas9-DA), which induces a single nucleotide conversion in the genome. Cas9-DA was introduced into sea urchin eggs with sgRNAs targeted for SpAlx1, SpDsh, or SpPks, each of which is critical for skeletogenesis, embryonic axis formation, or pigment formation, respectively. We found that both Cas9 and Cas9-DA edit the genome, and cause predicted phenotypic changes at a similar efficiency. Cas9, however, resulted in significant deletions in the genome centered on the gRNA target sequence, whereas Cas9-DA resulted in single or double nucleotide editing of C to T conversions within the gRNA target sequence.
conclusionsThese results suggest that the Cas9-DA approach may be useful for manipulating gene activity with decreased risks of genomic aberrations. Developmental Dynamics 246:1036-1046, 2017. © 2017 Wiley Periodicals, Inc.
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