ArticleMicrobiology (Reading, England)2017
Discovery of a novel lantibiotic nisin O from Blautia obeum A2-162, isolated from the human gastrointestinal tract.
Article in Microbiology (Reading, England), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 3 of them syntheses that pooled it.
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Who cites it
68 citing papers in PubMed, 3 syntheses or guidelines pooled it, 112 citations in OpenAlex.
- What is the impact of microbiota on dry eye: a literature review of the gut-eye axis.BMC ophthalmology · 2024Pooled it
- Checkpoint inhibitor responses can be regulated by the gut microbiota - A systematic review.Neoplasia (New York, N.Y.) · 2023Pooled it
- A systematic review of gut microbiome and ocular inflammatory diseases: Are they associated?Indian journal of ophthalmology · 2021Pooled it
- Temporal dynamics of gut biosynthetic gene clusters link persistent colonization and engraftment in fecal microbiota transplantation.Gut microbes · 2026Article
- Gut microbiota signatures associated with the development of Salmonella Typhimurium super-shedders in broiler chickens.Poultry science · 2026Article
- The Hungate1000 prokaryotic culture collection encodes a wide variety of bacteriocins.mSystems · 2026Article
- A comparative framework of natural nisin variants: linking genetic basis to future bioengineering applications.Microbial cell factories · 2026Review
- Associations of high protein supplements with gut microbiota and skeletal muscle mass in hospitalized older people.BMC geriatrics · 2025Article
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- MediaMatch: Prediction of Bacterial Growth on Different Culture Media Using the XGBoost Algorithm.Microbial biotechnology · 2025Article
- Changes in Probiotic Lachnospiraceae Genera Across Different Stages of COVID-19: A Meta-Analysis of 16S rRNA Microbial Data.Microorganisms · 2025Article
- Dissecting the Genetic Correlations and Causal Effects of Gut Microbiota on Pan-Cancer Phenotype: Driven by Common Dietary Preferences.Food science & nutrition · 2025Article
- Exploring the bioactive landscape: peptides and non-peptides from the human microbiota.NPJ biofilms and microbiomes · 2025Review
- A gut-derivedmBio · 2025Article
- The nisin O cluster: species dissemination, candidate leader peptide proteases and the role of regulatory systems.Microbiology (Reading, England) · 2025Article
- Article
- New approaches to secondary metabolite discovery from anaerobic gut microbes.Applied microbiology and biotechnology · 2025Review
- Mining metagenomic data to gain a new insight into the gut microbial biosynthetic potential in placental mammals.Microbiology spectrum · 2024Article
- Colorectal cancer microbiome programs DNA methylation of host cells by affecting methyl donor metabolism.Genome medicine · 2024Article
- Isolation of potentially novel species expands the genomic and functional diversity of Lachnospiraceae.iMeta · 2024Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
A novel lanC-like sequence was identified from the dominant human gut bacterium Blautia obeum strain A2-162. This sequence was extended to reveal a putative lantibiotic operon with biosynthetic and transport genes, two sets of regulatory genes, immunity genes, three identical copies of a nisin-like lanA gene with an unusual leader peptide, and a fourth putative lanA gene. Comparison with other nisin clusters showed that the closest relationship was to nisin U. B. obeum A2-162 demonstrated antimicrobial activity against Clostridium perfringens when grown on solid medium in the presence of trypsin. Fusions of predicted nsoA structural sequences with the nisin A leader were expressed in Lactococcus lactis containing the nisin A operon without nisA. Expression of the nisA leader sequence fused to the predicted structural nsoA1 produced a growth defect in L. lactis that was dependent upon the presence of biosynthetic genes, but failed to produce antimicrobial activity. Insertion of the nso cluster into L. lactis MG1614 gave an increased immunity to nisin A, but this was not replicated by the expression of nsoI. Nisin A induction of L. lactis containing the nso cluster and nisRK genes allowed detection of the NsoA1 pre-peptide by Western hybridization. When this heterologous producer was grown with nisin induction on solid medium, antimicrobial activity was demonstrated in the presence of trypsin against C. perfringens, Clostridium difficile and L. lactis. This research adds to evidence that lantibiotic production may be an important trait of gut bacteria and could lead to the development of novel treatments for intestinal diseases.
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