ArticleFood and environmental virology2018
Estimation of Human Norovirus Infectivity from Environmental Water Samples by In Situ Capture RT-qPCR Method.
Article in Food and environmental virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Rapid and accurate detection of infectious SARS-CoV-2 by viral receptor capture combined with loop-mediated isothermal amplification.Virologica Sinica · 2025Article
- Exploration of synthesis of anthocyanins in passion fruit pericarp through combinatorial transcriptomic analysis and WGCNA.Frontiers in plant science · 2025Article
- Environmental surveillance for Salmonella Typhi in rivers and wastewater from an informal sewage network in Blantyre, Malawi.PLoS neglected tropical diseases · 2024Article
- Fully Integrated Microfluidic Platform for Multiplexed Detection of Hunov by a Dynamic Confined-Space-Implemented One-Pot Rpa-Lamp System.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein Vaccine.Vaccines · 2023Article
- Persistence of Human Norovirus (GII) in Surface Water: Decay Rate Constants and Inactivation Mechanisms.Environmental science & technology · 2023Article
- Passive sampling to scale wastewater surveillance of infectious disease: Lessons learned from COVID-19.The Science of the total environment · 2022Review
- Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse.Frontiers in microbiology · 2022Article
- Advances and Future Perspective on Detection Technology of Human Norovirus.Pathogens (Basel, Switzerland) · 2021Review
- Moore swab performs equal to composite and outperforms grab sampling for SARS-CoV-2 monitoring in wastewater.The Science of the total environment · 2021Article
- Detection and disinfection of COVID-19 virus in wastewater.Environmental chemistry letters · 2021Review
- Human Intestinal Enteroids to Evaluate Human Norovirus GII.4 Inactivation by Aged-Green Tea.Frontiers in microbiology · 2020Article
- Characterization of Norovirus and Other Human Enteric Viruses in Sewage and Stool Samples Through Next-Generation Sequencing.Food and environmental virology · 2019Article
- Current and Emerging Technologies for the Detection of Norovirus from Shellfish.Foods (Basel, Switzerland) · 2019Article
- Infectivity and RNA Persistence of a Norovirus Surrogate, the Tulane Virus, in Oysters.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Human noroviruses (HuNoVs) are highly infectious viruses for which water is an important medium of transmission. In this study, we explored a new in situ capture RT-qPCR (ISC-RT-qPCR) methodology to estimate the infectivity of HuNoV in environmental water samples. This assay was based on capturing encapsidated HuNoV by viral receptors, followed by in situ amplification of the captured viral genomes by RT-qPCR. We demonstrated that the ISC-RT-qPCR did not capture and enable signal amplification of heat-denatured Tulane Virus (TV) and HuNoVs. We further demonstrated that the sensitivity of ISC-RT-qPCR was equal or better than that of conventional RT-qPCR procedures for the detection of HuNoV GI and GII. We then utilized the ISC-RT-qPCR to detect HuNoV in environmental water samples for comparison against that from a conventional RT-qPCR procedure. TV was used as a process control virus. While complete inhibition of TV genomic signal was observed in 27% of samples tested by RT-qPCR, no inhibition of TV genomic signal was observed by ISC-RT-qPCR. From 72 samples tested positive for HuNoV GI signal by RT-qPCR, only 20 (27.8%) of these samples tested positive by ISC-RT-qPCR, suggesting that 72.2% of RT-qPCR-positive samples were unlikely to be infectious. From 16 samples tested positive for HuNoV GII signal by RT-qPCR, only one of these samples tested positive by ISC-RT-qPCR. Five samples that had initially tested negative for HuNoV GII signal by RT-qPCR, was tested as positive by ISC-RT-qPCR. Overall, ISC-RT-qPCR method provided an alternative assay to estimate infectivity of HuNoV in environmental samples.
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Registered trials
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