ArticleBiomedical optics express2017
Plastic embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging.
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Long-range circuit of the mouse spinal trigeminal nucleus caudalis.The journal of headache and pain · 2026Article
- A discussion on the application of fluorescence micro-optical sectioning tomography in the research of cognitive dysfunction in diabetes.Open medicine (Warsaw, Poland) · 2025Article
- 3D Visualization of Whole Brain Vessels and Quantification of Vascular Pathology in a Chronic Hypoperfusion Model Causing White Matter Damage.Translational stroke research · 2024Article
- FCE-Net: a fast image contrast enhancement method based on deep learning for biomedical optical images.Biomedical optics express · 2022Article
- Simultaneous amplification of multiple immunofluorescence signals via cyclic staining of target molecules using mutually cross-adsorbed antibodies.Scientific reports · 2022Article
- Multiscale reconstruction of various vessels in the intact murine liver lobe.Communications biology · 2022Article
- High-Resolution Digital Panorama of Multiple Structures in Whole Brain of Alzheimer's Disease Mice.Frontiers in neuroscience · 2022Article
- Plastic embedding for precise imaging of large-scale biological tissues labeled with multiple fluorescent dyes and proteins.Biomedical optics express · 2021Article
- Dl-3-N-Butylphthalide Promotes Angiogenesis in an Optimized Model of Transient Ischemic Attack in C57BL/6 Mice.Frontiers in pharmacology · 2021Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-resolution three-dimensional biomolecule distribution information of large samples is essential to understanding their biological structure and function. Here, we proposed a method combining large sample resin embedding with iDISCO immunofluorescence staining to acquire the profile of biomolecules with high spatial resolution. We evaluated the compatibility of plastic embedding with an iDISCO staining technique and found that the fluorophores and the neuronal fine structures could be well preserved in the Lowicryl HM20 resin, and that numerous antibodies and fluorescent tracers worked well upon Lowicryl HM20 resin embedding. Further, using fluorescence Micro-Optical sectioning tomography (fMOST) technology combined with ultra-thin slicing and imaging, we were able to image the immunolabeled large-volume tissues with high resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.