ArticlePlant methods2017
Kinetic modelling: an integrated approach to analyze enzyme activity assays.
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Phytochemical analysis and enzyme inhibition of Origanum dictamnus L essential oil through in vitro and in silico methods.Scientific reports · 2025Article
- NNKcat: deep neural network to predict catalytic constants (Kcat) by integrating protein sequence and substrate structure with enhanced data imbalance handling.Briefings in bioinformatics · 2025Article
- Analysis of phosphofructokinase-1 activity as affected by pH and ATP concentration.Scientific reports · 2024Article
- Engineered repeat proteins as scaffolds to assemble multi-enzyme systems for efficient cell-free biosynthesis.Nature communications · 2023Article
- Protein scaffolds: A tool for multi-enzyme assembly.Biotechnology reports (Amsterdam, Netherlands) · 2021Review
- Extraction and Purification of Extracellular L-Glutamate Oxidase fromArchives of Razi Institute · 2021Article
- Enzyme Models-From Catalysis to Prodrugs.Molecules (Basel, Switzerland) · 2021Review
- A cost-effective enzyme kinetics and inhibition model for biochemistry education and research.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular BiologyArticle
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Authors and funding
4 authors.
Funding
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Abstract
backgroundIn general, enzyme activity is estimated from spectrophotometric data, by taking the slope of the linear part of the progress curve describing the rate of change in the substrate or product monitored. As long as the substrate concentrations are sufficiently high to saturate the enzyme and, the velocity of the catalyzed reaction is directly proportional to the enzyme concentration. Under these premises, this velocity can be taken as a measure of the amount of active enzyme present. Estimation of the enzyme activity through linear regression of the data should only be applied when linearity is true, which is often not the case or has not been checked.
resultsIn this paper, we propose a more elaborate method, based on a kinetic modelling approach, to estimate the in vitro specific enzyme activity from spectrophotometric assay data. As a case study, kinetic models were developed to estimate the activity of the enzymes pyruvate decarboxylase and alcohol dehydrogenase extracted from 'Jonagold' apple (
conclusionsIn contrast to the linear regression approach, the models can be used to estimate the enzyme activity regardless of whether linearity is achieved since they integrally take into account the complete progress curve. The use of kinetic models to estimate the enzyme activity can be applied to all other enzymes as long as the underlying reaction mechanism is known. The kinetic models can also be used as a tool to optimize the enzyme assays by systematically studying the effect of the various design parameters.
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