ArticleScientific reports2017
CRISPR/Cas9-mediated mutagenesis of the dihydroflavonol-4-reductase-B (DFR-B) locus in the Japanese morning glory Ipomoea (Pharbitis) nil.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 147 citations in OpenAlex.
- CRISPR/Cas9-mediated DFR disruption suggests coordinated changes in flavonoid flux and development in Petunia × hybrida.Plant cell reports · 2026Article
- Engineering plant architecture in the ornamental species Eustoma grandiflorum by knockout of strigolactone biosynthesis.Plant cell reports · 2026Article
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- A review of flavonoids at the crossroads of plant defense: integrating biotic and abiotic stress tolerance through AI- and CRISPR/Cas-guided metabolic reprogramming.Frontiers in plant science · 2026Review
- The road less taken: Dihydroflavonol 4-reductase inactivation and delphinidin anthocyanin loss underpins a natural intraspecific flower colour variation.Molecular ecology · 2025Article
- Advancing ornamental plant breeding through genomic technologies: opportunities, challenges, and future directions.Functional & integrative genomics · 2025Review
- CRISPR mediated gene editing for economically important traits in horticultural crops: progress and prospects.Transgenic research · 2025Review
- Resequencing and Functional Analysis Revealed ThatInternational journal of molecular sciences · 2025Article
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- Application of genome editing in plant reproductive biology: recent advances and challenges.Plant reproduction · 2024Review
- Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species.Plant cell reports · 2024Review
- Improvement of crop production in controlled environment agriculture through breeding.Frontiers in plant science · 2024Review
- Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update.Plants (Basel, Switzerland) · 2023Review
- Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis.Antioxidants (Basel, Switzerland) · 2023Review
- Genome-Wide Identification and Expression Analysis of ANS Family in Strawberry Fruits at Different Coloring Stages.International journal of molecular sciences · 2023Article
- Review
- The economics and policy of genome editing in crop improvement.The plant genome · 2023Review
- Biotechnologically Engineered Plants.Biology · 2023Review
- Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications.Frontiers in plant science · 2023Review
- Anthocyanin metabolic engineering ofFrontiers in plant science · 2023Review
1 more citing papers are in PubMed but not listed here.
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6 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas9 technology is a versatile tool for targeted mutagenesis in many organisms, including plants. However, this technique has not been applied to the Japanese morning glory (Ipomoea [Pharbitis] nil), a traditional garden plant chosen for the National BioResource Project in Japan. We selected dihydroflavonol-4-reductase-B (DFR-B) of I. nil, encoding an anthocyanin biosynthesis enzyme, as the target gene, and changes in the stem colour were observed during the early stages of plant tissue culture by Rhizobium [Agrobacterium]-mediated transformation. Twenty-four of the 32 (75%) transgenic plants bore anthocyanin-less white flowers with bi-allelic mutations at the Cas9 cleavage site in DFR-B, exhibiting a single base insertion or deletions of more than two bases. Thus, these results demonstrate that CRISPR/Cas9 technology enables the exploration of gene functions in this model horticultural plant. To our knowledge, this report is the first concerning flower colour changes in higher plants using CRISPR/Cas9 technology.
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