ArticleScientific reports2017
Highly efficient DNA-free gene disruption in the agricultural pest Ceratitis capitata by CRISPR-Cas9 ribonucleoprotein complexes.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 93 citations in OpenAlex.
- Article
- CRISPR-Cas9 mediated knockout of the white gene in the bluetongue virus vector, Culicoides sonorensis (biting midge).Scientific reports · 2026Article
- Chromosome-scale genome assembly of the South American fruit fly, Anastrepha fraterculus sp.1.Insect science · 2026Article
- DsiRNA-mediated silencing of Ceratitis capitata transformer or transformer-2 leads to masculinization of XX embryos and systemic gene silencing in ovaries.Insect science · 2026Article
- CRISPR/Cas9-mediated mutagenesis of the white-eye gene in the tephritid pest Bactrocera zonata.Insect science · 2026Article
- Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae).Insect science · 2026Article
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- Article
- CRISPR/Cas9 Genome Editing in the Diamondback Moth: Current Progress, Challenges, and Prospects.International journal of molecular sciences · 2025Review
- CRISPR/Cas9-mediated genome editing technique to control fall armyworm (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata.BMC biotechnology · 2024Article
- Gene drive and genetic sex conversion in the global agricultural pest Ceratitis capitata.Nature communications · 2024Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- CRISPR/Cas9 mediated editing of3 Biotech · 2023Article
- Role of Genes in Regulating Host Plants Expansion in Tephritid Fruit Flies (Diptera) and Potential for RNAi-Based Control.Journal of insect science (Online) · 2022Review
- In toto light sheet fluorescence microscopy live imaging datasets of Ceratitis capitata embryonic development.Scientific data · 2022Article
- CRISPR-mediated knockout of cardinal and cinnabar eye pigmentation genes in the western tarnished plant bug.Scientific reports · 2022Article
- Gene Editing and Genetic Control of Hemipteran Pests: Progress, Challenges and Perspectives.Frontiers in bioengineering and biotechnology · 2022Review
- Biotechnological Approaches for Host Plant Resistance to Insect Pests.Frontiers in genetics · 2022Review
Corrections and comments
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Authors and funding
20 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Mediterranean fruitfly Ceratitis capitata (medfly) is an invasive agricultural pest of high economic impact and has become an emerging model for developing new genetic control strategies as an alternative to insecticides. Here, we report the successful adaptation of CRISPR-Cas9-based gene disruption in the medfly by injecting in vitro pre-assembled, solubilized Cas9 ribonucleoprotein complexes (RNPs) loaded with gene-specific single guide RNAs (sgRNA) into early embryos. When targeting the eye pigmentation gene white eye (we), a high rate of somatic mosaicism in surviving G0 adults was observed. Germline transmission rate of mutated we alleles by G0 animals was on average above 52%, with individual cases achieving nearly 100%. We further recovered large deletions in the we gene when two sites were simultaneously targeted by two sgRNAs. CRISPR-Cas9 targeting of the Ceratitis ortholog of the Drosophila segmentation paired gene (Ccprd) caused segmental malformations in late embryos and in hatched larvae. Mutant phenotypes correlate with repair by non-homologous end-joining (NHEJ) lesions in the two targeted genes. This simple and highly effective Cas9 RNP-based gene editing to introduce mutations in C. capitata will significantly advance the design and development of new effective strategies for pest control management.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.