ArticleScientific reports2017
Improved vessel painting with carbocyanine dye-liposome solution for visualisation of vasculature.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Fluorescent Labeling Methods for Brain Structure Research.Molecules (Basel, Switzerland) · 2026Review
- Small Molecule Drug C381 Attenuates Brain Vascular Damage Following Repetitive Mild Traumatic Injury.Neurotrauma reports · 2024Article
- A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution.Cell reports methods · 2023Article
- Simple methodology to visualize whole-brain microvasculature in three dimensions.Neurophotonics · 2021Article
- TISSUE CLEARING.Nature reviews. Methods primers · 2021Article
- Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures.Scientific reports · 2020Article
- Using miniature brain implants in rodents for novel drug discovery.Expert opinion on drug discovery · 2019Review
- Optical clearing potential of immersion-based agents applied to thick mouse brain sections.PloS one · 2019Article
- Aberration correction considering curved sample surface shape for non-contact two-photon excitation microscopy with spatial light modulator.Scientific reports · 2018Article
- A Novel Technique for Visualizing and Analyzing the Cerebral Vasculature in Rodents.Translational stroke research · 2018Article
- Aqueous-based tissue clearing in crustaceans.Zoological letters · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vessel painting is one of the most accessible and cost-effective techniques for visualising vasculature by fluorescence microscopy. In this method, the hydrophobic carbocyanine dye DiIC18 labels the plasma membrane via insertion of its alkyl chains into the lipid bilayer. A major disadvantage of this procedure is that it does not stain veins and some microvessels in mouse brain. Furthermore, DiIC18 molecules can aggregate during perfusion, thereby occluding arteries and reducing the success rate and reproducibility of the experiment. To overcome these problems, we developed an improved vessel painting procedure that employs neutral liposomes (NLs) and DiIC12. NLs prevented DiI aggregation under physiological conditions whereas DiIC12 showed enhanced dye incorporation into liposomes and consequently increased staining intensity. Using this method, we successfully labelled all major blood vessel types in the mouse brain, including both veins and microvessels. Thus, liposome-mediated vessel painting is a simple and efficient method for visualising vasculature.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.