Evidence map›Paper›PMID 28855527›Full record

ArticleNature communications2017

Asymmetric dynamics of DNA entering and exiting a strongly confining nanopore.

Nicholas A W Bell, Kaikai Chen, Sandip Ghosal, Maria Ricci, Ulrich F Keyser

Abstract read
In one paragraph

Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Article
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  5. Article
  6. Review
  7. DNA Barcodes Using a Dual Nanopore Device.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  8. Article
  9. Review
  10. Article
  11. Review
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Monolithic Fabrication of NPN/SiNAdvanced materials interfaces · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nicholas A W BellDepartment of Physics, Cavendish Laboratory, Cambridge University, CB3 0HE, Cambridge, UK.
Kaikai ChenDepartment of Physics, Cavendish Laboratory, Cambridge University, CB3 0HE, Cambridge, UK.ORCID http://orcid.org/0000-0003-3170-0336
Sandip GhosalDepartment of Mechanical Engineering and Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, IL, 60208, USA.
Maria RicciDepartment of Physics, Cavendish Laboratory, Cambridge University, CB3 0HE, Cambridge, UK.
Ulrich F KeyserDepartment of Physics, Cavendish Laboratory, Cambridge University, CB3 0HE, Cambridge, UK. ufk20@cam.ac.uk.ORCID http://orcid.org/0000-0003-3188-5414

Funding

European Research Council 261101
6 · The paper itself

Abstract

In nanopore sensing, changes in ionic current are used to analyse single molecules in solution. The translocation dynamics of polyelectrolytes is of particular interest given potential applications such as DNA sequencing. In this paper, we determine how the dynamics of voltage driven DNA translocation can be affected by the nanopore geometry and hence the available configurational space for the DNA. Using the inherent geometrical asymmetry of a conically shaped nanopore, we examine how DNA dynamics depends on the directionality of transport. The total translocation time of DNA when exiting the extended conical confinement is significantly larger compared to the configuration where the DNA enters the pore from the open reservoir. By using specially designed DNA molecules with positional markers, we demonstrate that the translocation velocity progressively increases as the DNA exits from confinement. We show that a hydrodynamic model can account for these observations.Translocation of a charged polymer through confined nanoenvironments is highly dependent on their geometrical parameters. Here, the authors investigate experimentally the translocation dynamics of DNA through conical nanopores and provide a quantitative model for the translocation into and out of confinement.

Indexed as

Biological TransportDNAHydrodynamicsKineticsNanoporesDNA

Identifiers

PMID28855527
PMCPMC5577289

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.