ArticleMolecular biology of the cell2017
Shear stress induces noncanonical autophagy in intestinal epithelial monolayers.
Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Cellular membrane protein MipA from E.coli Nissle 1917 protects against Salmonella infection.The ISME journal · 2026Article
- Review
- The Fluidic Shear Stress Loading Method Enables Mechanobiological Stimulation in an On-Chip Pump-Integrated Microphysiological System.Micromachines · 2025Article
- Small Intestine on a Chip Demonstrates Physiologic Mucus Secretion in the Presence of Lacticaseibacillus rhamnosus Biofilm.Biotechnology and bioengineering · 2025Article
- Review
- An open-source, battery-powered, low-cost, and dual-channel pneumatic pulse generator for microfluidic cell-stretch assays.HardwareX · 2024Article
- Transmembrane channel-like 4 and 5 proteins at microvillar tips are potential ion channels and lipid scramblases.bioRxiv : the preprint server for biology · 2024Article
- Mechanical regulation of lipid and sugar absorption by Piezo1 in enterocytes.Acta pharmaceutica Sinica. B · 2024Article
- Activating autophagy promotes skin regeneration induced by mechanical stretch during tissue expansion.Burns & trauma · 2024Article
- Fluid flow to mimic organ function in 3DAPL bioengineering · 2023Review
- TrpA1 is a shear stress mechanosensing channel regulating intestinal stem cell proliferation inScience advances · 2023Article
- Microenvironmental elements singularity synergistically regulate the behavior and chemosensitivity of endometrioid carcinoma.Human cell · 2023Article
- Shear stress induces autophagy in Schlemm's canal cells via primary cilia-mediated SMAD2/3 signaling pathway.Autophagy reports · 2023Article
- Effects of Porous Size and Membrane Pattern on Shear Stress Characteristic in Gut-on-a-Chip with Peristalsis Motion.Micromachines · 2022Article
- Gq Signaling in Autophagy Control: Between Chemical and Mechanical Cues.Antioxidants (Basel, Switzerland) · 2022Review
- Mechanical Forces Govern Interactions of Host Cells with Intracellular Bacterial Pathogens.Microbiology and molecular biology reviews : MMBR · 2022Review
- How autophagy controls the intestinal epithelial barrier.Autophagy · 2022Review
- Autophagy and the primary cilium in cell metabolism: What's upstream?Frontiers in cell and developmental biology · 2022Review
- Magnetically-propelled fecal surrogates for modeling the impact of solid-induced shear forces on primary colonic epithelial cells.Biomaterials · 2021Article
- Low intermittent flow promotes rat mesenchymal stem cell differentiation in logarithmic fluid shear device.Biomicrofluidics · 2020Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
Flow of fluids through the gut, such as milk from a neonatal diet, generates a shear stress on the unilaminar epithelium lining the lumen. We report that exposure to physiological levels of fluid shear stress leads to the formation of large vacuoles, containing extracellular contents within polarizing intestinal epithelial cell monolayers. These observations lead to two questions: how can cells lacking primary cilia transduce shear stress, and what molecular pathways support the formation of vacuoles that can exceed 80% of the cell volume? We find that shear forces are sensed by actin-rich microvilli that eventually generate the apical brush border, providing evidence that these structures possess mechanosensing ability. Importantly, we identified the molecular pathway that regulates large vacuole formation downstream from mechanostimulation to involve central components of the autophagy pathway, including ATG5 and LC3, but not Beclin. Together our results establish a novel link between the actin-rich microvilli, the macroscopic transport of fluids across cells, and the noncanonical autophagy pathway in organized epithelial monolayers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.