Evidence map›Paper›PMID 28854867›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2018

An Embryonic and Induced Pluripotent Stem Cell Model for Ovarian Granulosa Cell Development and Steroidogenesis.

Shane Lipskind, Jennifer S Lindsey, Behzad Gerami-Naini, Jennifer L Eaton, Daniel O'Connell, Adam Kiezun, Joshua W K Ho, Nicholas Ng, Parveen Parasar, Michelle Ng and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. NAM journal · 2025
    Review
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Identification of Stem Cell-Like Cells in the Ovary.Tissue engineering and regenerative medicine · 2022
    Review
  14. Article
  15. Review
  16. Pluripotent Cell Models for Gonadal Research.International journal of molecular sciences · 2019
    Review
  17. Review
  18. Review
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Shane Lipskind1 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Jennifer S Lindsey1 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Behzad Gerami-Naini2 Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Jennifer L Eaton2 Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Daniel O'Connell2 Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Adam Kiezun3 Computational Methods Development, Cancer Genome Analysis, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Joshua W K Ho4 Victor Chang Cardiac Research Institute, University of New South Wales, Sydney, New South Wales, Australia.
Nicholas Ng1 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Parveen Parasar1 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Michelle Ng1 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Michael Nickerson1 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Utkan Demirci5 Canary Center at Stanford for Early Cancer Detection, Stanford School of Medicine, Palo Alto, CA, USA.
Richard Maas2 Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Raymond M Anchan1 Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

Funding

Development of Scholars in Ob-Gyn for the 21th CenturyK12HD001255 · NICHD · BRIGHAM AND WOMEN'S HOSPITAL · PI BARBIERI, ROBERT L · 1999 to 2009
$2.6M
Minimizing the role of cryoprotectant toxicity for cryopreservationR01EB015776 · NIBIB · STANFORD UNIVERSITY · PI DEMIRCI, UTKAN · 2012 to 2015
$1.5M
NIBIB NIH HHS R01 EB015776NICHD NIH HHS K12 HD001255
6 · The paper itself

Abstract

Embryoid bodies (EBs) can serve as a system for evaluating pluripotency, cellular differentiation, and tissue morphogenesis. In this study, we use EBs derived from mouse embryonic stem cells (mESCs) and human amniocyte-derived induced pluripotent stem cells (hAdiPSCs) as a model for ovarian granulosa cell (GC) development and steroidogenic cell commitment. We demonstrated that spontaneously differentiated murine EBs (mEBs) and human EBs (hEBs) displayed ovarian GC markers, such as aromatase (CYP19A1), FOXL2, AMHR2, FSHR, and GJA1. Comparative microarray analysis identified both shared and unique gene expression between mEBs and the maturing mouse ovary. Gene sets related to gonadogenesis, lipid metabolism, and ovarian development were significantly overrepresented in EBs. Of the 29 genes, 15 that were differentially regulated in steroidogenic mEBs displayed temporal expression changes between embryonic, postnatal, and mature ovarian tissues by polymerase chain reaction. Importantly, both mEBs and hEBs were capable of gonadotropin-responsive estradiol (E2) synthesis in vitro (217-759 pg/mL). Live fluorescence-activated cell sorting-sorted AMHR2

Indexed as

AnimalsEmbryoid BodiesEmbryonic Stem CellsFemaleGene ExpressionGranulosa CellsHumansInduced Pluripotent Stem CellsMiceSteroidsSteroidsembryonic stem cellsiPSCnative hormonesovarian tissue regenerationsteroidogenesis

Identifiers

PMID28854867
PMCPMC6344958

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.