ArticleFEMS microbiology letters2017
Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates.
Article in FEMS microbiology letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Industrial applicability of enzymatic and whole-cell processes for the utilization of C1 building blocks.Nature communications · 2025Review
- Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases.Chemical reviews · 2024Review
- A multicomponent propane monooxygenase catalyzes the initial degradation of methylApplied and environmental microbiology · 2023Article
- Crucial Role of the Chaperonin GroES/EL for Heterologous Production of the Soluble Methane Monooxygenase from Methylomonas methanica MC09.Chembiochem : a European journal of chemical biology · 2022Article
- Research progress in bioremediation of petroleum pollution.Environmental science and pollution research international · 2021Review
- Biochemistry of aerobic biological methane oxidation.Chemical Society reviews · 2021Review
- Heterologous Expression of Mycobacterium Alkene Monooxygenases in Gram-Positive and Gram-Negative Bacterial Hosts.Applied and environmental microbiology · 2018Article
- Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b.Methods in enzymology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Soluble methane monooxygenase (sMMO) from methane-oxidising bacteria can oxygenate more than 100 hydrocarbons and is one of the most catalytically versatile biological oxidation catalysts. Expression of recombinant sMMO has to date not been achieved in Escherichia coli and so an alternative expression system must be used to manipulate it genetically. Here we report substantial improvements to the previously described system for mutagenesis of sMMO and expression of recombinant enzymes in a methanotroph (Methylosinus trichosporium OB3b) expression system. This system has been utilised to make a number of new mutants and to engineer sMMO to increase its catalytic precision with a specific substrate whilst increasing activity by up to 6-fold. These results are the first 'proof-of-principle' experiments illustrating the feasibility of developing sMMO-derived catalysts for diverse applications.
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Registered trials
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