Evidence map›Paper›PMID 28852897›Full record

ArticleInternational ophthalmology2018

Effect of berberine on lipopolysaccharide-induced monocyte chemotactic protein-1 and interleukin-8 expression in a human retinal pigment epithelial cell line.

Hu-Shan Cui, Yu-Min Li, Wei Fang, Jiu-Ke Li, Yuan-Min Dai, Lian-Shun Zheng

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International ophthalmology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Natural Compounds for Preventing Age-Related Diseases and Cancers.International journal of molecular sciences · 2024
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Hu-Shan CuiDepartment of Ophthalmology, Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, No. 3 Qingchun Road East, Hangzhou, 310016, Zhejiang Province, China. cuihushan@hotmail.com.ORCID http://orcid.org/0000-0003-2237-4153
Yu-Min LiDepartment of Ophthalmology, Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, No. 3 Qingchun Road East, Hangzhou, 310016, Zhejiang Province, China.
Wei FangDepartment of Ophthalmology, Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, No. 3 Qingchun Road East, Hangzhou, 310016, Zhejiang Province, China.
Jiu-Ke LiDepartment of Ophthalmology, Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, No. 3 Qingchun Road East, Hangzhou, 310016, Zhejiang Province, China.
Yuan-Min DaiDepartment of Ophthalmology, Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, No. 3 Qingchun Road East, Hangzhou, 310016, Zhejiang Province, China.
Lian-Shun ZhengInstitute of Anatomy and Cell Biology, School of Medicine, Zhejiang University, Hangzhou, China.
Sir Run Run Shaw Hospital · CNZhejiang University · CN

Funding

Zhejiang Province Medical and health science and technology project 2014KYA104Zhejiang Province Traditional Chinese medicine science and technology planning project 2016ZA132
6 · The paper itself

Abstract

purposeIn this study, we elucidated the effects of berberine, a major alkaloid component contained in medicinal herbs, such as Phellodendri Cortex and Coptidis Rhizoma, on expression of monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) in a human retinal pigment epithelial cell line (ARPE-19) caused by lipopolysaccharide (LPS) stimulation.

methodsARPE-19 cells were cultured to confluence. Berberine and LPS were added to the medium. MCP-1 and IL-8 mRNA were measured by real-time polymerase chain reaction. MCP-1 and IL-8 protein concentrations in the media were measured using enzyme-linked immunosorbent assay.

resultsAfter stimulation with LPS, MCP-1 and IL-8 mRNA in ARPE-19 cells reached maximum levels at 3 h, and MCP-1 and IL-8 protein in the culture media reached maximum levels at 24 h. Berberine dose-dependently inhibited MCP-1 and IL-8 mRNA expression of the cells and protein levels in the media stimulated with LPS.

conclusionsThese findings indicate that berberine inhibited the expression of MCP-1 and IL-8 induced by LPS.

Indexed as

BerberineCells, CulturedChemokine CCL2Enzyme-Linked Immunosorbent AssayGene Expression RegulationHumansInterleukin-8LipopolysaccharidesMacular DegenerationPigment Epithelium of EyeRNABerberineCCL2 protein, humanChemokine CCL2CXCL8 protein, humanInterleukin-8LipopolysaccharidesRNABerberineHuman retinal pigment epithelial cellsInterleukin-8LipopolysaccharideMonocyte chemotactic protein-1

Identifiers

PMID28852897
PMCPMC6153896
OpenAlexW2748074843

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.