Evidence map›Paper›PMID 28852171›Full record

ArticleScientific reports2017

Integrative Approach with Electrophysiological and Theoretical Methods Reveals a New Role of S4 Positively Charged Residues in PKD2L1 Channel Voltage-Sensing.

Tomohiro Numata, Kunichika Tsumoto, Kazunori Yamada, Tatsuki Kurokawa, Shinichi Hirose, Hideki Nomura, Mitsuhiro Kawano, Yoshihisa Kurachi, Ryuji Inoue, Yasuo Mori

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

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  7. Opening TRPP2 (Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Tomohiro NumataDepartment of Physiology, Graduate School of Medical Sciences, Fukuoka University, 7-45-1 Nanakuma, Johnan-ku, Fukuoka, Fukuoka, 814-0180, Japan. numata@fukuoka-u.ac.jp.
Kunichika TsumotoDepartment of Pharmacology, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan.ORCID 0000-0001-7457-3125
Kazunori YamadaDivision of Rheumatology, Department of Internal Medicine, Kanazawa University Graduate School of Medicine, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan.
Tatsuki KurokawaLaboratory of Molecular Biology, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Kyoto, 615-8510, Japan.
Shinichi HiroseDepartment of Pediatrics School of Medicine, Fukuoka University, 7-45-1 Nanakuma, Johnan-ku, Fukuoka, Fukuoka, 814-0180, Japan.
Hideki NomuraDepartment of General Medicine, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan.
Mitsuhiro KawanoDivision of Rheumatology, Department of Internal Medicine, Kanazawa University Graduate School of Medicine, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan.
Yoshihisa KurachiDepartment of Pharmacology, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan.
Ryuji InoueDepartment of Physiology, Graduate School of Medical Sciences, Fukuoka University, 7-45-1 Nanakuma, Johnan-ku, Fukuoka, Fukuoka, 814-0180, Japan. inouery@fukuoka-u.ac.jp.
Yasuo MoriLaboratory of Molecular Biology, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Kyoto, 615-8510, Japan. mori@sbchem.kyoto-u.ac.jp.
Kyoto University · JPFukuoka University · JPKanazawa University · JPThe University of Osaka · JPKanazawa University Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Numerical model-based simulations provide important insights into ion channel gating when experimental limitations exist. Here, a novel strategy combining numerical simulations with patch clamp experiments was used to investigate the net positive charges in the putative transmembrane segment 4 (S4) of the atypical, positively-shifted voltage-dependence of polycystic kidney disease 2-like 1 (PKD2L1) channel. Charge-neutralising mutations (K452Q, K455Q and K461Q) in S4 reduced gating charges, positively shifted the Boltzmann-type activation curve [i.e., open probability (P

Indexed as

Amino AcidsAmino Acid SubstitutionCalcium ChannelsModels, MolecularMutant ProteinsMutation, MissensePatch-Clamp TechniquesReceptors, Cell SurfaceAmino AcidsCalcium ChannelsMutant ProteinsPKD2L1 protein, humanReceptors, Cell Surface

Identifiers

PMID28852171
PMCPMC5575081
OpenAlexW2747880862

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.