ArticleScientific reports2017
Label-free optical vibrational spectroscopy to detect the metabolic state of M. tuberculosis cells at the site of disease.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Visualizing Metabolism: Lipid-Based Raman Nanoreporters as an Enabling Platform for Metabolic Imaging and Precision Medicine.ACS nano medicine · 2026Review
- Monitoring Live Mycobacteria in Real-Time Using a Microfluidic Acoustic-Raman Platform.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Implications of drug-induced phenotypical resistance: Is isoniazid radicalizingFrontiers in antibiotics · 2022Article
- Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform.Communications biology · 2020Article
- Modelling the effects of bacterial cell state and spatial location on tuberculosis treatment: Insights from a hybrid multiscale cellular automaton model.Journal of theoretical biology · 2018Article
- Ultrastructural Analysis of Cell Envelope and Accumulation of Lipid Inclusions in ClinicalFrontiers in microbiology · 2017Article
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Authors and funding
7 authors.
Funding
Abstract
Tuberculosis relapse is a barrier to shorter treatment. It is thought that lipid rich cells, phenotypically resistant to antibiotics, may play a major role. Most studies investigating relapse use sputum samples although tissue bacteria may play an important role. We developed a non-destructive, label-free technique combining wavelength modulated Raman (WMR) spectroscopy and fluorescence detection (Nile Red staining) to interrogate Mycobacterium tuberculosis cell state. This approach could differentiate single "dormant" (lipid rich, LR) and "non-dormant" (lipid poor, LP) cells with high sensitivity and specificity. We applied this to experimentally infected guinea pig lung sections and were able to distinguish both cell types showing that the LR phenotype dominates in infected tissue. Both in-vitro and ex-vivo spectra correlated well, showing for the first time that Mycobacterium tuberculosis, likely to be phenotypically resistant to antibiotics, are present in large numbers in tissue. This is an important step in understanding the pathology of relapse supporting the idea that they may be caused by M. tuberculosis cells with lipid inclusions.
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