ArticleScientific reports2017
A multi-trap microfluidic chip enabling longitudinal studies of nerve regeneration in Caenorhabditis elegans.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Integrated Microfluidic Platform for Longitudinal Imaging, Functional Assays, and Downstream Proteomics in C. elegans.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Difference in Intestine Content ofMicromachines · 2023Article
- High-resolution imaging and analysis of the intestinal bacterial load ofRSC advances · 2023Article
- The impact of selected abiotic factors on Artemia hatching process through real-time observation of oxygen changes in a microfluidic platform.Scientific reports · 2023Article
- Femtosecond laser microdissection for isolation of regenerating C. elegans neurons for single-cell RNA sequencing.Nature methods · 2023Article
- A self-purifying microfluidic system for identifying drugs acting against adult schistosomes.Royal Society open science · 2022Article
- CDK14 Promotes Axon Regeneration by Regulating the Noncanonical Wnt Signaling Pathway in a Kinase-Independent Manner.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Microfluidic-based imaging of complete Caenorhabditis elegans larval development.Development (Cambridge, England) · 2021Article
- Article
- Stress and timing associated withHeliyon · 2020Article
- An automated microfluidic device for time-lapse imaging of mouse embryonic stem cells.Biomicrofluidics · 2019Article
- Digging deeper: methodologies for high-content phenotyping in Caenorhabditis elegans.Lab animal · 2019Review
- Automated high-content phenotyping from the first larval stage till the onset of adulthood of the nematode Caenorhabditis elegans.Lab on a chip · 2018Article
- High-Content Microfluidic Screening Platform Used To Identify σ2R/Tmem97 Binding Ligands that Reduce Age-Dependent Neurodegeneration in C. elegans SC_APP Model.ACS chemical neuroscience · 2018Article
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7 authors.
Funding
Abstract
Several sophisticated microfluidic devices have recently been proposed for femtosecond laser axotomy in the nematode C. elegans for immobilization of the animals for surgery to overcome time-consuming and labor-intensive manual processes. However, nerve regeneration studies require long-term recovery of the animals and multiple imaging sessions to observe the regeneration capabilities of their axons post-injury. Here we present a simple, multi-trap device, consisting of a single PDMS (polydimethylsiloxane) layer, which can immobilize up to 20 animals at the favorable orientation for optical access needed for precise laser surgery and high-resolution imaging. The new device, named "worm hospital" allows us to perform the entire nerve regeneration studies, including on-chip axotomy, post-surgery housing for recovery, and post-recovery imaging all on one microfluidic chip. Utilizing the worm hospital and analysis of mutants, we observed that most but not all neurodevelopmental genes in the Wnt/Frizzled pathway are important for regeneration of the two touch receptor neurons ALM and PLM. Using our new chip, we observed that the cwn-2 and cfz-2 mutations significantly reduced the reconnection possibilities of both neurons without any significant reduction in the regrowth lengths of the severed axons. We observed a similar regeneration phenotype with cwn-1 mutation in ALM neurons only.
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