ArticleScientific reports2017
Fast free-of-acrylamide clearing tissue (FACT)-an optimized new protocol for rapid, high-resolution imaging of three-dimensional brain tissue.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Rapid sonication-assisted whole tissue clearing and immunostaining.Scientific reports · 2025Article
- Distinct origin and region-dependent contribution of stromal fibroblasts to fibrosis following traumatic injury in mice.Nature neuroscience · 2024Article
- A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy.International journal of molecular sciences · 2023Review
- Enhanced characterization of beta cell mass in a Tg(BioImpacts : BI · 2022Article
- Automated optimization of endoderm differentiation on chip.Lab on a chip · 2021Article
- Light-Sheet Microscopy of the Optic Nerve Reveals Axonal Degeneration and Microglial Activation in NMDA-Induced Retinal Injury.EC ophthalmology · 2021Article
- Characterization of Astrocyte Morphology and Function Using a Fast and Reliable Tissue Clearing Technique.Current protocols · 2021Article
- Nicotine induces morphological and functional changes in astrocytes via nicotinic receptor activity.Glia · 2021Article
- Three-dimensional remodeling of functional cerebrovascular architecture and gliovascular unit in leptin receptor-deficient mice.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Article
- Developmental distribution of primary cilia in the retinofugal visual pathway.The Journal of comparative neurology · 2021Article
- Tissue clearing technique: Recent progress and biomedical applications.Journal of anatomy · 2021Review
- Application of Airy beam light sheet microscopy to examine early neurodevelopmental structures in 3D hiPSC-derived human cortical spheroids.Molecular autism · 2021Article
- Tissue Optical Clearing for Biomedical Imaging: From In Vitro to In Vivo.Advances in experimental medicine and biology · 2021Article
- Three-Dimensional Imaging in Stem Cell-Based Researches.Frontiers in veterinary science · 2021Review
- Conventional histomorphometry and fast free of acrylamide clearing tissue (FACT) visualization of sciatic nerve in chicken (Veterinary research forum : an international quarterly journal · 2021Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
- Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy.Neuron · 2020Review
- HSP70/IL-2 Treated NK Cells Effectively Cross the Blood Brain Barrier and Target Tumor Cells in a Rat Model of Induced Glioblastoma Multiforme (GBM).International journal of molecular sciences · 2020Article
- Tissue clearing and its applications in neuroscience.Nature reviews. Neuroscience · 2020Review
- Tools and Approaches for Studying MicrogliaFrontiers in immunology · 2020Review
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Authors and funding
8 authors.
Funding
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Abstract
Fast Free-of-Acrylamide Clearing Tissue (FACT) is a new sodium dodecyl sulfate (SDS)-based clearing protocol for the chemical clearing and imaging of brain tissue containing transgenic or immunolabeled fluorescent proteins. In the present study, we have developed this new method and optimized multiple dimensions of the workflow, including reduced clearing time, improved efficiency of fluorescent signals without the need for electrophoretic or complex instrumentations, preservation of cytoarchitectural details, optimized confocal microscopy, and accelerated data collection. We systematically compared seven clearing protocols with the FACT protocol, using transgenic mouse brains with fluorochrome expression in microglia. Only six days were required for detecting transgene-labeled markers in a 1-mm thick brain slice from adult mice, and 14 days were required for detecting antibody-labeled markers in the same-sized tissue. Preservation of fluorescent signal was achieved by decreasing clearing time, adjusting the pH of the SDS solution, and using the appropriate temperature for tissue clearing, all of which contributed to the superiority of our method. We conclude that the FACT protocol can be successfully applied to the fluorescent imaging of mouse brain tissue, and will facilitate structural analyses and connectomics of large assemblies of cells and their networks in the context of three-dimensional organ systems.
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