Evidence map›Paper›PMID 28851864›Full record

ArticleNature communications2017

Improved split fluorescent proteins for endogenous protein labeling.

Siyu Feng, Sayaka Sekine, Veronica Pessino, Han Li, Manuel D Leonetti, Bo Huang

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 172 papers.

0numbers the graph read from it
0cells of the map it votes in
172citing papers in PubMed
22.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

172 citing papers in PubMed, 290 citations in OpenAlex.

  1. Article
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  3. Efficient Endogenous Tagging in the Sea Urchin,bioRxiv : the preprint server for biology · 2026
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  4. Design of Fluorescent Membrane Scaffold Proteins for Nanodiscs.bioRxiv : the preprint server for biology · 2026
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  10. Computational design of membrane fusion proteins.bioRxiv : the preprint server for biology · 2026
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  11. Transsynaptic linking of calcium channels and postsynaptic receptors at a dyadic synapse.Proceedings of the National Academy of Sciences of the United States of America · 2026
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  16. Review
  17. Heparan sulfate is essential for Drosophila FGF export.bioRxiv : the preprint server for biology · 2026
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  18. Article
  19. bioRxiv : the preprint server for biology · 2026
    Article
  20. Article

112 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Siyu FengThe UC Berkeley-UCSF Graduate Program in Bioengineering, San Francisco, CA, 94143, USA.
Sayaka SekineDepartment of Pharmaceutical Chemistry, University of California in San Francisco, San Francisco, CA, 94143, USA.
Veronica PessinoGraduate Program of Biophysics, University of California, San Francisco, San Francisco, CA, 94143, USA.
Han LiDepartment of Cellular and Molecular Pharmacology, and Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, 94143, USA.
Manuel D LeonettiDepartment of Cellular and Molecular Pharmacology, and Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, 94143, USA.
Bo HuangDepartment of Pharmaceutical Chemistry, University of California in San Francisco, San Francisco, CA, 94143, USA. bo.huang@ucsf.edu.ORCID http://orcid.org/0000-0003-1704-4141
University of California, San Francisco · USHoward Hughes Medical Institute · USUniversity of California, Berkeley · US

Funding

STRUCTURAL BIOLOGY TRAINING PROGRAMT32GM008284 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KORTEMME, TANJA · 1988 to 2022
$10.1M
Solving macromolecular complex architecture in situ by super-resolution microscopDP2OD008479 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUANG, BO · 2011 to 2011
$2.3M
Multimerized GFP probe for live cell imagingR21EB022798 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUANG, BO · 2016 to 2017
$436k
NIBIB NIH HHS R21 EB022798NIGMS NIH HHS T32 GM008284NIH HHS DP2 OD008479
6 · The paper itself

Abstract

Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell-cell contact. To expand this toolset, we have developed a screening strategy for the direct engineering of self-complementing split FPs. Via this strategy, we have generated a yellow-green split-mNeonGreen2

Indexed as

Protein EngineeringEndoplasmic ReticulumHEK293 CellsHumansLuminescent ProteinsMicroscopy, FluorescenceProtein TransportSEC Translocation ChannelsLuminescent ProteinsSEC61B protein, humanSEC Translocation Channels

Identifiers

PMID28851864
PMCPMC5575300
OpenAlexW2950985576

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.