ArticleBMC cell biology2017
Coral cell separation and isolation by fluorescence-activated cell sorting (FACS).
Article in BMC cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 69 citations in OpenAlex.
- Exploring different methods of Exaiptasia diaphana infection to follow Vibrio parahaemolyticus dissemination in the whole animal.BMC microbiology · 2025Article
- Cell type-specific immune regulation under symbiosis in a facultatively symbiotic coral.The ISME journal · 2025Article
- Candidate stem cell isolation and transplantation in Hexacorallia.Cell reports · 2024Article
- Saving coral reefs: significance and biotechnological approaches for coral conservation.Advanced biotechnology · 2024Review
- Fluorescent proteins generate a genetic color polymorphism and counteract oxidative stress in intertidal sea anemones.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Robust global arrangement by coherent enhancement in Huygens-Fresnel traveling surface acoustic wave interference field.Analytical and bioanalytical chemistry · 2024Article
- A targeted approach to enrich host-associated bacteria for metagenomic sequencing.FEMS microbes · 2024Article
- Corals have algal friends for dinner.Nature · 2023Article
- Application of a Magnetic Platform in α6 Integrin-Positive iPSC-TM Purification.Bioengineering (Basel, Switzerland) · 2023Article
- Impact of rising seawater temperature on a phagocytic cell population duringFrontiers in immunology · 2023Article
- Methods for cell isolation and analysis of the highly regenerative tunicateFrontiers in cell and developmental biology · 2023Article
- Effects of Ocean Warming on the Underexplored Members of the Coral Microbiome.Integrative and comparative biology · 2022Review
- Harnessing the Power of Model Organisms To Unravel Microbial Functions in the Coral Holobiont.Microbiology and molecular biology reviews : MMBR · 2022Review
- Article
- Heat stress increases immune cell function in Hexacorallia.Frontiers in immunology · 2022Article
- Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Intracellular Bacterial Symbionts in Corals: Challenges and Future Directions.Microorganisms · 2021Review
- Intracellular pH regulation: characterization and functional investigation of HBMC molecular and cell biology · 2021Article
- Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundGeneralized methods for understanding the cell biology of non-model species are quite rare, yet very much needed. In order to address this issue, we have modified a technique traditionally used in the biomedical field for ecological and evolutionary research. Fluorescent activated cell sorting (FACS) is often used for sorting and identifying cell populations. In this study, we developed a method to identify and isolate different cell populations in corals and other cnidarians.
methodsUsing fluorescence-activated cell sorting (FACS), coral cell suspension were sorted into different cellular populations using fluorescent cell markers that are non-species specific. Over 30 different cell markers were tested. Additionally, cell suspension from Aiptasia pallida was also tested, and a phagocytosis test was done as a downstream functional assay.
resultsWe found that 24 of the screened markers positively labeled coral cells and 16 differentiated cell sub-populations. We identified 12 different cellular sub-populations using three markers, and found that each sub-population is primarily homogeneous. Lastly, we verified this technique in a sea anemone, Aiptasia pallida, and found that with minor modifications, a similar gating strategy can be successfully applied. Additionally, within A. pallida, we show elevated phagocytosis of sorted cells based on an immune associated marker.
conclusionsIn this study, we successfully adapted FACS for isolating coral cell populations and conclude that this technique is translatable for future use in other species. This technique has the potential to be used for different types of studies on the cellular stress response and other immunological studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.