Evidence map›Paper›PMID 28850078›Full record

ArticleMolecules (Basel, Switzerland)2017

Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position.

Wei Liu, Dongwon Shin, Martin Ng, Karissa Y Sanbonmatsu, Yitzhak Tor, Barry S Cooperman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Isomorphic Fluorescent Nucleosides.Accounts of chemical research · 2024
    Article
  3. Article
  4. Ataluren and aminoglycosides stimulate read-through of nonsense codons by orthogonal mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Wei LiuDepartment of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA. weiliu@sas.upenn.edu.
Dongwon ShinDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA. dshin@trilinkbiotech.com.
Martin NgDepartment of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA. martinng@sas.upenn.edu.
Karissa Y SanbonmatsuTheoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. kys@lanl.gov.
Yitzhak TorDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA. ytor@ucsd.edu.
Barry S CoopermanDepartment of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA. cooprman@pobox.upenn.edu.

Funding

Large-Scale Simulations of Ribosomal DecodingR01GM072686 · NIGMS · UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB · PI SANBONMATSU, KARISSA Y · 2005 to 2024
$5.5M
Fluorescent Nucleosides and OligonucleotidesR01GM069773 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TOR, YITZHAK · 2004 to 2020
$5.0M
Single Molecule Dynamics of mRNA TranslationR01GM080376 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI COOPERMAN, BARRY S., GOLDMAN, YALE E · 2008 to 2016
$2.5M
NIGMS NIH HHS R01 GM069773NIGMS NIH HHS R01 GM072686NIGMS NIH HHS R01 GM080376
6 · The paper itself

Abstract

Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon University of California base formation during protein synthesis. Our results suggest that codon:anticodon base pairing is subject to tighter constraints at the middle position than at the 5'- and 3'-positions, and further suggest a sequential mechanism of formation of the three base pairs in the codon:anticodon helix.

Indexed as

Base PairingGenetic CodeKineticsModels, MolecularNucleotidesRibosomesRNA, MessengerRNA, TransferNucleotidesRNA, MessengerRNA, Transferatomic mutagenesisfluorescent mRNAkinetic mechanismmRNA:tRNA base pairingsteric constraint

Identifiers

PMID28850078
PMCPMC5753802

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.