Evidence map›Paper›PMID 28849480›Full record

ArticleAdvances in experimental medicine and biology

HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens.

Antonio Francioso, Sergio Fanelli, Daniele Vigli, Laura Ricceri, Rosaria A Cavallaro, Alessia Baseggio Conrado, Mario Fontana, Maria D'Erme, Luciana Mosca

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In one paragraph

Article in Advances in experimental medicine and biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Antonio FranciosoDepartment of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, Piazzale Aldo Moro, 00185, Rome, Italy. antonio.francioso@uniroma1.it.
Sergio FanelliDepartment of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, Piazzale Aldo Moro, 00185, Rome, Italy.
Daniele VigliDepartment of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, Piazzale Aldo Moro, 00185, Rome, Italy.
Laura RicceriDepartment of Cell Biology and Neuroscience, Istituto Superiore di Sanità, Viale Regina Elena, Rome, Italy.
Rosaria A CavallaroDepartment of Surgery "P. Valdoni", Sapienza University of Rome, Via Antonio Scarpa, 14, 00161, Rome, Italy.
Alessia Baseggio ConradoPhotobiology Unit, University of Dundee, Ninewells Hospital and School of Medicine, Dundee, DD1 9SY, UK.
Mario FontanaDepartment of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, Piazzale Aldo Moro, 00185, Rome, Italy.
Maria D'ErmeDepartment of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, Piazzale Aldo Moro, 00185, Rome, Italy.
Luciana MoscaDepartment of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, Piazzale Aldo Moro, 00185, Rome, Italy.
Sapienza University of Rome · ITIstituto Superiore di Sanità · ITNinewells Hospital · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There is an increasing interest for analytical methods aimed to detect biological sulfur-containing amines, because of their involvement in human diseases and metabolic disorders. This work describes an improved HPLC method for the determination of sulfur containing amino acids and amines from different biological matrices. We optimized a pre-column derivatization procedure using dabsyl chloride, in which dabsylated products can be monitored spectrophotometrically at 460 nm. This method allows the simultaneous analysis of biogenic amines, amino acids and sulfo-amino compounds including carnosine, dopamine, epinephrine, glutathione, cysteine, taurine, lanthionine, and cystathionine in brain specimens, urines, plasma, and cell lysates. Moreover, the method is suitable for the study of physiological and non-physiological derivatives of taurine and glutathione such as hypotaurine, homotaurine, homocysteic acid and S-acetylglutathione. The present method displays good efficiency of derivatization, having the advantage to give rise to stable products compared to other derivatizing agents such as o-phthalaldehyde and dansyl chloride.With this method, we provide a tool to study sulfur cycle from a metabolic point of view in relation to the pattern of biological amino-compounds, allowing researchers to get a complete scenario of organic sulfur and amino metabolism in tissues and cells.

Indexed as

Amino AcidsAnimalsBiogenic AminesChromatography, High Pressure LiquidHumansMiceSulfur CompoundsAmino AcidsBiogenic AminesSulfur CompoundsDabsyl chlorideHigh pressure/performance liquid chromatographySulfur-containing bioactive compounds

Identifiers

PMID28849480
OpenAlexW2747398537

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.