Evidence map›Paper›PMID 28849283›Full record

ArticleArchives of virology2017

Phylogenetic analysis of VP1 and RdRP genes of Brazilian aichivirus B strains involved in a diarrhea outbreak in dairy calves.

Juliane Ribeiro, Elis Lorenzetti, José Carlos Ribeiro Júnior, Thais Neris da Silva Medeiros, Alice Fernandes Alfieri, Amauri Alcindo Alfieri

Abstract read
In one paragraph

Article in Archives of virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Severe outbreak of bovine neonatal diarrhea in a dairy calf rearing unit with multifactorial etiology.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021
    Article
  3. Proposed New Strain of CanineMicrobiology resource announcements · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Juliane RibeiroLaboratory of Animal Virology, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, CEP 86057-970, Londrina, PR, Brazil.
Elis LorenzettiMulti-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, CEP 86057-970, Londrina, PR, Brazil.
José Carlos Ribeiro JúniorMulti-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, CEP 86057-970, Londrina, PR, Brazil.
Thais Neris da Silva MedeirosLaboratory of Animal Virology, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, CEP 86057-970, Londrina, PR, Brazil.
Alice Fernandes AlfieriMulti-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, CEP 86057-970, Londrina, PR, Brazil.
Amauri Alcindo AlfieriLaboratory of Animal Virology, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10011, CEP 86057-970, Londrina, PR, Brazil. alfieri@uel.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aichivirus B has been reported worldwide in calves and adult cattle with and without diarrhea. The aim of this study was to describe the molecular characteristics of the RdRP and VP1 genes of aichivirus B strains identified as the most frequent etiologic agent in a neonatal diarrhea outbreak in a high-production Brazilian dairy cattle herd. Preliminary laboratory analysis ruled out important enteropathogens (Cryptosporidium spp; Eimeria spp., E. coli F5, and bovine coronavirus). Fecal samples from diarrheic (n = 24) and asymptomatic (n = 5) calves up to 30 days old were collected for virological analysis. RT-PCR assays were performed for the detection of aichivirus B RdRP and VP1 genes and for rotavirus A VP7 and VP4 genes in fecal samples. Asymptomatic calves (control group) were negative for both viruses. Aichivirus B and rotavirus A G10P[11] genotypes were found in 54.2% (13/24) and 25% (6/24) of the diarrheic fecal samples, respectively. Aichivirus B was only identified (83.3%, 10/12) in calves up to two weeks old. Phylogenetic analysis based on the RdRP gene grouped the Brazilian strains in a new branch within the aichivirus B group. Comparative analysis of the nucleotide sequence of the VP1 gene of Brazilian and Chinese aichivirus B strains allowed the strains identified in this study to be classified in the putative lineage 1. This is the first description of a high rate of aichivirus B detection in a diarrhea outbreak in dairy calves, and the first phylogenetic study of the VP1 gene of aichivirus B wild-type strains performed in South America.

Indexed as

Disease OutbreaksPhylogenyAnimalsBrazilCattleCattle DiseasesDiarrheaKobuvirusPicornaviridae InfectionsReverse Transcriptase Polymerase Chain ReactionRNA-Dependent RNA PolymeraseSequence HomologyViral Structural ProteinsRNA-Dependent RNA PolymeraseViral Structural Proteins

Identifiers

PMID28849283
PMCPMC7086745

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.