ArticleMolecular medicine reports2017
Adhesion, proliferation and osteogenic differentiation of mesenchymal stem cells in 3D printed poly-ε-caprolactone/hydroxyapatite scaffolds combined with bone marrow clots.
Article in Molecular medicine reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Synthesis and characterization of 3D gelatin/calcium hydroxide/dentin matrix proteins/propolis scaffolds for hard tissue engineering.BMC oral health · 2025Article
- Preventing MSC aging and enhancing immunomodulation: Novel strategies for cell-based therapies.Regenerative therapy · 2025Review
- 3D Bioprinting in Limb Salvage Surgery.Journal of functional biomaterials · 2024Review
- Nickel-titanium alloy porous scaffolds based on a dominant cellular structure manufactured by laser powder bed fusion have satisfactory osteogenic efficacy.Materials today. Bio · 2024Article
- A cost-effective and enhanced mesenchymal stem cell expansion platform with internal plasma-activated biofunctional interfaces.Materials today. Bio · 2023Article
- Development of Nonwoven Fibrous Materials Based on Poly-3-Hydroxybutyrate with a High Content of α-Tricalcium Phosphate.Polymers · 2023Article
- Outcome Analysis of Osseous Ingrowth in an Artificially Created Gap Non-union Using the Novel 3D Biodegradable Polycaprolactone Poly-l-Lactide Polymer Scaffold: Insights from an Experimental Study.Indian journal of orthopaedics · 2022Article
- Novel In Situ-Cross-Linked Electrospun Gelatin/Hydroxyapatite Nonwoven Scaffolds Prove Suitable for Periodontal Tissue Engineering.Pharmaceutics · 2022Article
- Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation.Scientific reports · 2021Article
- Investigation of 3D-Printed Polycaprolactone-/Polyvinylpyrrolidone-Based Constructs.Cartilage · 2021Article
- The Application of Polycaprolactone in Three-Dimensional Printing Scaffolds for Bone Tissue Engineering.Polymers · 2021Review
- Advances in 3D Printing for Tissue Engineering.Materials (Basel, Switzerland) · 2021Review
- Mesenchymal stem cells in the treatment of osteonecrosis of the jaw.Journal of the Korean Association of Oral and Maxillofacial Surgeons · 2021Review
- Breast Cancer Cells Metastasize to the Tissue-Engineered Premetastatic Niche by Using an Osteoid-Formed Polycaprolactone/Nanohydroxyapatite Scaffold.Computational and mathematical methods in medicine · 2021Article
- Highly Porous 3D Printed Tantalum Scaffolds Have Better Biomechanical and Microstructural Properties than Titanium Scaffolds.BioMed research international · 2021Article
- Preparation and biological properties of silk fibroin/nano-hydroxyapatite/graphene oxide scaffolds with an oriented channel-like structure.RSC advances · 2020Article
- A Rabbit Model of Osteochondral Regeneration Using Three-Dimensional Printed Polycaprolactone-Hydroxyapatite Scaffolds Coated with Umbilical Cord Blood Mesenchymal Stem Cells and Chondrocytes.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- PCL-Based Composite Scaffold Matrices for Tissue Engineering Applications.Molecular biotechnology · 2018Review
- Progress of Regenerative Therapy in Orthopedics.Current osteoporosis reports · 2018Review
- Recent Advances in Biomaterials for 3D Printing and Tissue Engineering.Journal of functional biomaterials · 2018Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cells (MSCs), a stem cell population capable of multi‑lineage differentiation, bound to porous biomaterial scaffolds, are widely used for bone tissue regeneration. However, there is evidence to suggest that MSC collection from bone marrow and expansion in vitro may result in phenotypic changes including a loss of differentiation potential and cell senescence. The aim of the present study was to find a facile and efficient approach to enable MSC adhesion and proliferation to scaffolds with osteogenic differentiation. Unprocessed bone marrow blood from the condyle of the distal femur in the rabbits were added to three‑dimensional (3D) printed porous poly-ε-caprolactone/hydroxyapatite (PCL/HA) scaffolds with bone marrow clots (MC) formed, using two different methods for Group A (MC enriched scaffolds) and Group B (MC combined scaffolds), and then were cultured in osteogenic medium for 4 weeks. The scaffolds were assessed macroscopically and microscopically. Scaffold bioactivity and the proliferation and osteogenic differentiation of seeded MSCs were measured. Higher cellular viability and greater cell numbers in the scaffolds at later phases of culture were observed in Group B compared with Group A. In addition, Group B was associated with greater osteoinductivity, alkaline phosphatase activity and bony nodule formation, as assessed using scanning electron microscopy. Furthermore, reverse transcription‑quantitative polymerase chain reaction analysis revealed that more osteogenic differentiation was present in Group B, compared with Group A. MC combined scaffolds proved to be a highly efficient, reliable and simple novel method for MSC adhesion, proliferation and differentiation. The MC combined PCL‑HA multi‑scale porosity scaffold may represent a candidate for future bone regeneration studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.