ArticleMolecular medicine reports2017
Methylation of mitochondrial DNA displacement loop region regulates mitochondrial copy number in colorectal cancer.
Article in Molecular medicine reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Preliminary Analysis of CpG, CHG and CHH Methylation in the Mitochondrial Control Region of Dogs With Malignant Mammary Gland Tumours.Animal genetics · 2026Article
- Mitochondrial D-Loop Region Methylation Is Not Altered in Children with Autism Spectrum Disorder.Epigenomes · 2026Article
- Mitochondria and tumorigenesis: Molecular basis and therapeutic implications.Genes & diseases · 2026Review
- Spectrum and Impact of Mitochondrial DNA Mutations in Ovarian Cancer.International journal of molecular sciences · 2025Review
- Study on Abnormal Angiogenesis in Moyamoya Disease via Mitochondrial D-Loop Methylation.Brain and behavior · 2025Article
- Rewriting nuclear epigenetic scripts in mitochondrial diseases as a strategy for heteroplasmy control.EMBO molecular medicine · 2025Article
- Oxidized Low-Density Lipoprotein as a Potential Target for Enhancing Immune Checkpoint Inhibitor Therapy in Microsatellite-Stable Colorectal Cancer.Antioxidants (Basel, Switzerland) · 2025Review
- Decoding the Methylome: Insights into the Epigenetic Regulation of Polycystic Ovarian Syndrome through Mitochondrial DNA Methylation.Current genomics · 2025Review
- Targeting the epigenome and tumor heterogeneity: advances in immunotherapy for chemoresistant metastatic colorectal cancer.Frontiers in immunology · 2025Review
- Mitochondrial epigenetic mechanisms in cancer: an updated overview.Frontiers in cell and developmental biology · 2025Review
- Novel mtDNA methylation-associated prognostic signatures in colorectal cancer.Frontiers in oncology · 2025Article
- Associations between Circulating Biomarkers of One-Carbon Metabolism and Mitochondrial D-Loop Region Methylation Levels.Epigenomes · 2024Article
- The mitoepigenome responds to stress, suggesting novel mito-nuclear interactions in vertebrates.BMC genomics · 2023Article
- Characterization of Mitochondrial DNA Methylation of Alzheimer's Disease in Plasma Cell-Free DNA.Diagnostics (Basel, Switzerland) · 2023Article
- Changes in Mitochondrial Epigenome in Type 2 Diabetes Mellitus.British journal of biomedical science · 2023Review
- Association between diesel exhaust exposure and mitochondrial DNA methylation.Carcinogenesis · 2022Article
- Insights regarding mitochondrial DNA copy number alterations in human cancer (Review).International journal of molecular medicine · 2022Review
- Article
- Decreased mitochondrial D-loop region methylation mediates an increase in mitochondrial DNA copy number in CADASIL.Clinical epigenetics · 2022Article
- Mitochondrial DNA Methylation and Human Diseases.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is not established whether de‑methylation of the displacement loop (D‑loop) region if mitochondrial DNA (mtDNA) directly influences mtDNA copy number and further alters the cell cycle, apoptosis and cell proliferation in colorectal cancer. The current study employed cell viability assays, cell cycle analysis, and mtDNA methylation analysis using 5 colorectal cancer cell lines. The present results demonstrated that 5‑aza‑2'‑deoxycytidine (5‑AZA), a DNA hypomethylating agent, significantly increased proliferation of Lovo and Colo‑205 colorectal cancer cell lines. In Colo‑205 cells, the proportion of G0/G1 phase cells was increased following 5‑AZA treatment. Additionally, the apoptosis rate in Colo‑205 cells was decreased by 5‑AZA treatment. Compared with their controls, a significantly higher mtDNA copy number was observed in Colo‑205 and Lovo cells following 5‑AZA treatment. Notably, the Colo‑205 and Lovo cells had relatively higher methylation levels at the 4 and 6th/7th CpG sites of D‑loop region, respectively, compared with the levels at the corresponding sites following 5‑AZA treatment. However, in HCT116, SW480, LS‑174T, and HT‑29 cells, 5‑AZA treatment did not induce a significant change in proliferation, cell cycle, apoptosis and mtDNA copy number. Demethylation at the 4 and 6th/7th CpG sites of the D‑loop region of HCT116, SW480, LS‑174T and HT‑29 cells was not observed following 5‑AZA treatment. In conclusion, de‑methylation of specific sites on CpG islands of D‑loop promoter may lead to the elevation of mtDNA copy number in colorectal cancer, triggering alterations in biological behaviors, including increased cell proliferation, reduced apoptosis and a relative cell cycle arrest in G0/G1 phase.
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