ArticleACS chemical biology2017
Structure and Functional Analysis of ClbQ, an Unusual Intermediate-Releasing Thioesterase from the Colibactin Biosynthetic Pathway.
Article in ACS chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Article
- Analysis of the partial sequencing of clbA, clbB and clbQ in Escherichia coli isolates that produce colibactin and multilocus sequence typing.Scientific reports · 2024Article
- Current understandings of colibactin regulation.Microbiology (Reading, England) · 2024Review
- Targeted depletion ofmSystems · 2023Article
- Multiple Functions of the Type II Thioesterase Associated with the Phoslactomycin Polyketide Synthase.Biochemistry · 2022Article
- Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains.Synthetic and systems biotechnology · 2022Review
- Thioesterase enzyme families: Functions, structures, and mechanisms.Protein science : a publication of the Protein Society · 2022Article
- "Effect of Subinhibitory Concentrations of Some Antibiotics and Low Doses of Gamma Radiation on the Cytotoxicity and Expression of Colibactin by an Uropathogenic Escherichia coli isolate".Current microbiology · 2021Article
- Biosynthesis of depsipeptides, or Depsi: The peptides with varied generations.Protein science : a publication of the Protein Society · 2020Review
- Structure and bioactivity of colibactin.Bioorganic & medicinal chemistry letters · 2020Review
- ClbR Is the Key Transcriptional Activator of Colibactin Gene Expression in Escherichia coli.mSphere · 2020Article
- The Polyamine Spermidine Modulates the Production of the Bacterial Genotoxin Colibactin.mSphere · 2019Article
- Synthesis and reactivity of precolibactin 886.Nature chemistry · 2019Article
- Macrocyclic colibactin induces DNA double-strand breaks via copper-mediated oxidative cleavage.Nature chemistry · 2019Article
- Structure elucidation of colibactin and its DNA cross-links.Science (New York, N.Y.) · 2019Article
- YbtT is a low-specificity type II thioesterase that maintains production of the metallophore yersiniabactin in pathogenic enterobacteria.The Journal of biological chemistry · 2018Article
- Model Colibactins Exhibit Human Cell Genotoxicity in the Absence of Host Bacteria.ACS chemical biology · 2018Article
- Colibactin: More Than a New Bacterial Toxin.Toxins · 2018Review
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Colibactin is a genotoxic hybrid nonribosomal peptide/polyketide secondary metabolite produced by various pathogenic and probiotic bacteria residing in the human gut. The presence of colibactin metabolites has been correlated to colorectal cancer formation in several studies. The specific function of many gene products in the colibactin gene cluster can be predicted. However, the role of ClbQ, a type II editing thioesterase, has not been established. The importance of ClbQ has been demonstrated by genetic deletions that abolish colibactin cytotoxic activity, and recent studies suggest an atypical role in releasing pathway intermediates from the assembly line. Here we report the 2.0 Å crystal structure and biochemical characterization of ClbQ. Our data reveal that ClbQ exhibits greater catalytic efficiency toward acyl-thioester substrates as compared to precolibactin intermediates and does not discriminate among carrier proteins. Cyclized pyridone-containing colibactins, which are off-pathway derivatives, are not viable substrates for ClbQ, while linear precursors are, supporting a role of ClbQ in facilitating the promiscuous off-loading of premature precolibactin metabolites and novel insights into colibactin biosynthesis.
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