Evidence map›Paper›PMID 28843507›Full record

ArticleProtein expression and purification2018

Purification of metal-dependent lysine deacetylases with consistently high activity.

Tasha B Toro, Richard G Painter, Rashad A Haynes, Elena Y Glotser, Melyssa R Bratton, Jenae R Bryant, Kyara A Nichols, Asia N Matthew-Onabanjo, Ashley N Matthew, Derek R Bratcher and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein expression and purification, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Tasha B ToroDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: ttoro@xula.edu.
Richard G PainterDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: rgpainter@gmail.com.
Rashad A HaynesDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: rhaynes1@xula.edu.
Elena Y GlotserDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: eglotser@xula.edu.
Melyssa R BrattonDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: mbratton@xula.edu.
Jenae R BryantDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: jbryant001@hotmail.com.
Kyara A NicholsDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: knichols@xula.edu.
Asia N Matthew-OnabanjoDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: asia.matthew2@umassmed.edu.
Ashley N MatthewDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: Ashley.matthew2@umassmed.edu.
Derek R BratcherDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: dbratch89@yahoo.com.
Chanel D PerryDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: chanelperry07@gmail.com.
Terry J WattDepartment of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, LA 70125-1098, USA. Electronic address: tjwatt@xula.edu.
Xavier University of Louisiana · US

Funding

Building Integrated Pathways to Independence for Diverse Biomedical ResearchersUL1GM118967 · NIGMS · XAVIER UNIVERSITY OF LOUISIANA · PI FOROOZESH, MARYAM, GIGUETTE, MARGUERITE · 2015 to 2023
$17.7M
Xavier's RCMI Cancer Research ProgramG12MD007595 · NIMHD · XAVIER UNIVERSITY OF LOUISIANA · PI D'AMOUR, GENE, WANG, GUANGDI · 2012 to 2018
$15.7M
PROJECT PATHWAYS: STUDENT TRAINING CORETL4GM118968 · NIGMS · XAVIER UNIVERSITY OF LOUISIANA · PI FOROOZESH, MARYAM, GIGUETTE, MARGUERITE · 2015 to 2023
$9.2M
Project Pathways: Research Enrichment CoreRL5GM118966 · NIGMS · XAVIER UNIVERSITY OF LOUISIANA · PI FOROOZESH, MARYAM, GIGUETTE, MARGUERITE · 2015 to 2023
$6.6M
NIGMS NIH HHS RL5 GM118966NIGMS NIH HHS TL4 GM118968NIGMS NIH HHS UL1 GM118967NIMHD NIH HHS G12 MD007595
6 · The paper itself

Abstract

Metal-dependent lysine deacetylases (KDACs) are involved in regulation of numerous biological and disease processes through control of post-translational acetylation. Characterization of KDAC activity and substrate identification is complicated by inconsistent activity of prepared enzyme and a range of multi-step purifications. We describe a simplified protocol based on two-step affinity chromatography. The purification method is appropriate for use regardless of expression host, and we demonstrate purification of several representative members of the KDAC family as well as a selection of mutated variants. The purified proteins are highly active and consistent across preparations.

Indexed as

AnimalsCircular DichroismCobaltElectrophoresis, Polyacrylamide GelEscherichia coliHistone DeacetylasesHumansRecombinant ProteinsRepressor ProteinsSf9 CellsCobaltHDAC8 protein, humanHistone DeacetylasesRecombinant ProteinsRepressor ProteinshdacHistone deacetylaseLysine deacetylase

Identifiers

PMID28843507
PMCPMC5624855
OpenAlexW2750033247

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.