ArticleKidney international2018
Single-tubule RNA-Seq uncovers signaling mechanisms that defend against hyponatremia in SIADH.
Article in Kidney international, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- Energy metabolism in the kidney and its role in chronic kidney disease.Clinical and experimental nephrology · 2026Review
- Druggability Studies of Benzene Sulfonamide Substituted Diarylamide (E3) as a Novel Diuretic.Biomedicines · 2025Article
- Roles of Insulin-Like Growth Factor-1 in Muscle Wasting and Osteopenia in Mice with Hyponatremia.Calcified tissue international · 2025Article
- Proteomics and AQP2 regulation.The Journal of physiology · 2024Review
- Lectin-mediated, time-efficient, and high-yield sorting of different morphologically intact nephron segments.Pflugers Archiv : European journal of physiology · 2024Article
- Role of paraoxonase 3 in regulating ENaC-mediated NaThe Journal of physiology · 2024Article
- Mild dehydration effects on the murine kidney single-nucleus transcriptome and chromatin accessibility.American journal of physiology. Renal physiology · 2023Article
- Effect of controlled hypotensive hemorrhage on plasma sodium levels in anesthetized pigs: An exploratory study.Physiological reports · 2023Article
- Aldosterone Contributes to Vasopressin Escape through Changes in Water and Urea Transport.Biomedicines · 2023Article
- Signaling mechanisms in renal compensatory hypertrophy revealed by multi-omics.Nature communications · 2023Article
- Insulin-regulated aminopeptidase is required for water excretion in response to acute hypotonic stress.American journal of physiology. Renal physiology · 2023Article
- Systems Biology of the Vasopressin V2 Receptor: New Tools for Discovery of Molecular Actions of a GPCR.Annual review of pharmacology and toxicology · 2022Review
- Editorial: Insights in renal and epithelial physiology: 2021.Frontiers in physiology · 2022Article
- Glucocorticoid Receptor Maintains Vasopressin Responses in Kidney Collecting Duct Cells.Frontiers in physiology · 2022Article
- Bayesian identification of candidate transcription factors for the regulation ofAmerican journal of physiology. Renal physiology · 2021Article
- Rapid development of vasopressin resistance in dietary KAmerican journal of physiology. Renal physiology · 2021Article
- Pax2 and Pax8 Proteins Regulate Urea Transporters and Aquaporins to Control Urine Concentration in the Adult Kidney.Journal of the American Society of Nephrology : JASN · 2020Article
- RNA-Seq and protein mass spectrometry in microdissected kidney tubules reveal signaling processes initiating lithium-induced nephrogenic diabetes insipidus.Kidney international · 2019Article
- Representation and relative abundance of cell-type selective markers in whole-kidney RNA-Seq data.Kidney international · 2019Article
- Vasopressin-aquaporin-2 pathway: recent advances in understanding water balance disorders.F1000Research · 2019Review
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
In the syndrome of inappropriate antidiuretic hormone secretion (SIADH), hyponatremia is limited by onset of vasopressin-escape caused by loss of the water channel aquaporin-2 in the renal collecting duct despite high circulating vasopressin. Here, we use the methods of systems biology in a well-established rat model of SIADH to identify signaling pathways activated at the onset of vasopressin-escape. Using single-tubule RNA-Seq, full transcriptomes were determined in microdissected cortical collecting ducts of vasopressin-treated rats at 1, 2, and 4 days after initiation of oral water loading in comparison to time-control rats without water loading. The time-dependent mRNA abundance changes were mapped to gene sets associated with curated canonical signaling pathways and revealed evidence of perturbation of transforming growth factor β signaling and epithelial-to-mesenchymal transition on Day 1 of water loading simultaneous with the initial fall in Aqp2 gene expression. On Day 2 of water loading, transcriptomic changes mapped to Notch signaling and the transition from G0 into the cell cycle but arrest at the G2/M stage. There was no evidence of cell proliferation or altered principal or intercalated cell numbers. Exposure of vasopressin-treated cultured mpkCCD cells to transforming growth factor β resulted in a virtually complete loss of aquaporin-2. Thus, there is a partial epithelial-to-mesenchymal transition during vasopressin escape with a subsequent shift from quiescence into the cell cycle with eventual arrest and loss of aquaporin-2.
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