ArticleJournal of assisted reproduction and genetics2017
Association between the MTHFR-C677T isoform and structure of sperm DNA.
Article in Journal of assisted reproduction and genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Polymorphisms of methalenetetrahydrofolate reductase in recurrent pregnancy loss: an overview of systematic reviews and meta-analyses.Journal of assisted reproduction and genetics · 2019Pooled it
- Health professionals require more defined protocols, better funding and patient resources to support couples with recurrent pregnancy loss.Scientific reports · 2025Article
- The Study ofInternational journal of fertility & sterility · 2025Article
- Variations in folate prescriptions for patients with the MTHFR genetic polymorphisms: A case series study.Exploratory research in clinical and social pharmacy · 2023Article
- Active Folate Versus Folic Acid: The Role of 5-MTHF (Methylfolate) in Human Health.Integrative medicine (Encinitas, Calif.) · 2022Article
- Association of paternal MTHFR polymorphisms (C677T) with clinical outcomes in ICSI treatment.Frontiers in endocrinology · 2022Article
- C677T polymorphism increases the risk of early spontaneous abortion.Journal of assisted reproduction and genetics · 2019Article
- Comparison of different types of twin pregnancies in terms of obstetric and perinatal outcomes: association of vanished twins with methylenetetrahydrofolate reductase (MTHFR) polymorphism(s).Journal of assisted reproduction and genetics · 2018Article
- Metabolic enhancers supporting 1-carbon cycle affect sperm functionality: an in vitro comparative study.Scientific reports · 2018Article
- MTHFR isoform carriers. 5-MTHF (5-methyl tetrahydrofolate) vs folic acid: a key to pregnancy outcome: a case series.Journal of assisted reproduction and genetics · 2018Article
- Evaluation of sperm chromatin structure and DNA strand breaks is an important part of clinical male fertility assessment.Translational andrology and urology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe aim of this study is to evaluate whether the MTHFR contribution to male decreased fertility can be attributable to anomalies in sperm nucleus DNA structure in relation to defective methylation.
methodsThe presence of MTHFR C677T, contributing at most for male infertility, was determined from a venous blood sample, using real-time polymerase chain reaction (PCR). Sperm DNA fragmentation (SDF) and sperm nucleus decondensation index (SDI) measurements were performed using acridine orange and flow cytometry. SDF and SDI of men MTHFR C677T heterozygous or homozygous were compared to a general population of hypo-fertile patients
resultsSDF is not increased either in homozygous or heterozygous carriers of MTHFR C677T. In contrast, SDI is increased with a higher incidence in homozygous (p = 0.0006) than in heterozygous (p = 0.029) patients when compared with the control population. Using a critical threshold of 20% for either SDI or SDF assayed with our technique, the percentage of patients with results higher than this value is not significant with respect to fragmentation (0.128), but is significantly increased for decondensation (0.0003).
conclusionsDefective methylation linked to MTHFR may contribute to sperm pathogenesis via increased SDI. After DNA structure analysis, especially SDI, treatment with 5-methyl tetrahydrofolate (MTHF), the metabolite downstream from the action of MTHFR, should be recommended as a therapeutic approach. Patients with a high SDI should be tested for MTHFR isoforms as part of a healthcare policy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.