ArticleCell stress & chaperones2018
Evaluation of DNA methylation and mRNA expression of heat shock proteins in thermal manipulated chicken.
Article in Cell stress & chaperones, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 83 citations in OpenAlex.
- Liver response to early embryonic stimulation with xylo-and mannooligosaccharides in broiler chickens.Journal of applied genetics · 2026Article
- Effects of variable incubation temperature during embryonic days 10-13 on leg muscle transcriptomics in day-old broiler and layer chicks.Scientific reports · 2026Article
- Methylation and expression dynamics in broiler liver followingJournal of veterinary research · 2026Article
- Mechanistic insights into avian and chiropteran thermal risk exposure in a warming world.Conservation physiology · 2026Review
- Short-term impacts of embryonic thermal manipulation in mule duck, a kinetic study: new tools for metabolic programming.BMC genomics · 2025Article
- Review
- Biological Mechanisms for Allen's Rule: DNA Methylation as Mediator of the Association Between In Utero Exposure to Environmental Heat and Tibial Growth in Childhood.American journal of human biology : the official journal of the Human Biology Council · 2025Article
- Impact of Thermal Manipulation of Broiler Eggs on Growth Performance, Splenic Inflammatory Cytokine Levels, and Heat Shock Protein Responses to Post-Hatch Lipopolysaccharide (LPS) Challenge.Animals : an open access journal from MDPI · 2025Article
- Molecular regulation of whole genome DNA methylation in heat stress response of dairy cows.BMC genomics · 2025Article
- Exploring Epigenetic and Genetic Modulation in Animal Responses to Thermal Stress.Molecular biotechnology · 2025Review
- Ambient outdoor heat and accelerated epigenetic aging among older adults in the US.Science advances · 2025Article
- Promoter Region and Regulatory Elements of IGF and VIP Genes Associated With Reproductive Traits in Chicken.International journal of genomics · 2025Article
- Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study.Environmental epigenetics · 2025Article
- Mini review: Studying epigenomic alterations can shed light on coping and adaptive abilities during heat stress in monogastric livestock.Frontiers in genetics · 2025Review
- Thermal Manipulation During the Embryonic Stage and the Post-Hatch Characteristics of Broiler Chickens.Animals : an open access journal from MDPI · 2024Article
- Effects of heat stress on global DNA methylation and blood parameters of two strains of laying hens.Tropical animal health and production · 2024Article
- Embryonic thermal manipulation: a potential strategy to mitigate heat stress in broiler chickens for sustainable poultry production.Journal of animal science and biotechnology · 2024Review
- To live or let die? Epigenetic adaptations to climate change-a review.Environmental epigenetics · 2024Review
- Embryonic manipulations shape life-long, heritable stress responses through complex epigenetic mechanisms: a review.Frontiers in neuroscience · 2024Review
- Transgenerational transmission of environmental effects in livestock in the age of global warming.Cell stress & chaperones · 2023Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thermal manipulation during embryogenesis has been demonstrated to enhance the thermotolerance capacity of broilers through epigenetic modifications. Heat shock proteins (HSPs) are induced in response to stress for guarding cells against damage. The present study investigates the effect of thermal conditioning during embryogenesis and thermal challenge at 42 days of age on HSP gene and protein expression, DNA methylation and in vitro luciferase assay in brain tissue of Naked Neck (NN) and Punjab Broiler-2 (PB-2) chicken. On the 15th day of incubation, fertile eggs from two breeds, NN and PB-2, were randomly divided in to two groups: control (C)-eggs were incubated under standard incubation conditions, and thermal conditioning (TC)-eggs were exposed to higher incubation temperature (40.5°C) for 3 h on the 15th, 16th, and 17th days of incubation. The chicks obtained from each group were further subdivided and reared under different environmental conditions from the 15th to the 42nd day as normal [N; 25 ± 1 °C, 70% relative humidity (RH)] and heat exposed (HE; 35 ± 1 °C, 50% RH) resulting in four treatment groups (CN, CHE, TCN, and TCHE). The results revealed that HSP promoter activity was stronger in CHE, which had lesser methylation and higher gene expression. The activity of promoter region was lesser in TCHE birds that were thermally manipulated at the embryonic stage, thus reflecting their stress-free condition. This was confirmed by the lower level of mRNA expression of all the HSP genes. In conclusion, thermal conditioning during embryogenesis has a positive impact and improves chicken thermotolerance capacity in postnatal life.
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