Evidence map›Paper›PMID 28842627›Full record

ArticleScientific reports2017

Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields.

Dinesh Parate, Alfredo Franco-Obregón, Jürg Fröhlich, Christian Beyer, Azlina A Abbas, Tunku Kamarul, James H P Hui, Zheng Yang

3 registry-linked trialsOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 3 registered trials, which are not on this map. Cited by 61 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
61citing papers in PubMed, 4 pooled it
6.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03944993 naterminatednot on this mapstarted 2019, after this paper: background citation

Effects of Pulsed Electromagnetic Fields (PEMFS) On Cerebral Haemodynamics - EPOCH Study

TypeinterventionalSponsorNational University Hospital, SingaporeRan2019 to 2021Enrolled6ConditionsCerebral Hemodynamics, Cognitive ChangeArmsPulsed Electromagnetic Fields Therapy (Dominant leg), Pulsed Electromagnetic Fields Therapy (Non-dominant leg), Sham Therapy (Control)
NCT04183543 naunknown statusnot on this mapstarted 2020, after this paper: background citation

PEMF Therapy for the Management of Diabetes in Obese Patients (ProMise): A Randomized Controlled Trial

TypeinterventionalSponsorNational University Hospital, SingaporeRan2020 to 2022Enrolled40ConditionsObesity, Type 2 DiabetesArmsPulsed Electromagnetic Fields, Sham Therapy
NCT05920746 nacompletednot on this mapstarted 2021, after this paper: background citation

Type of Magnetic Field and the Effects of Rheumatoid Arthritis Treatment

TypeinterventionalSponsorJolanta ZwolińskaRan2021 to 2023Enrolled72ConditionsRheumatoid Arthritis, Hand RheumatismArmsKinesiotherapy with magnetotherapy
3 · Its place in the literature

Who cites it

61 citing papers in PubMed, 4 syntheses or guidelines pooled it, 97 citations in OpenAlex.

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1 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Dinesh ParateDepartment of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, NUHS Tower Block, Level 11, 1E Kent Ridge Road, Singapore, 119288, Singapore.
Alfredo Franco-ObregónDepartment of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, NUHS Tower Block, Level 8, IE Kent Ridge Road, Singapore, 119228, Singapore. suraf@nus.edu.sg.
Jürg FröhlichDepartment of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, NUHS Tower Block, Level 8, IE Kent Ridge Road, Singapore, 119228, Singapore.ORCID 0000-0002-5923-3410
Christian BeyerInstitute for Electromagnetic Fields, Swiss Federal Institute of Technology (ETH), Rämistrasse 101, 8092, Zurich, Switzerland.
Azlina A AbbasTissue Engineering Group (TEG), National Orthopaedic Centre of Excellence for Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Pantai Valley, Kuala Lumpur, 50603, Malaysia.
Tunku KamarulTissue Engineering Group (TEG), National Orthopaedic Centre of Excellence for Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Pantai Valley, Kuala Lumpur, 50603, Malaysia.
James H P HuiDepartment of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, NUHS Tower Block, Level 11, 1E Kent Ridge Road, Singapore, 119288, Singapore. james_hui@nuhs.edu.sg.
Zheng YangDepartment of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, NUHS Tower Block, Level 11, 1E Kent Ridge Road, Singapore, 119288, Singapore. lsiyz@nus.edu.sg.
National University of Singapore · SGUniversity of Malaya · MYÉcole Polytechnique Fédérale de Lausanne · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pulse electromagnetic fields (PEMFs) have been shown to recruit calcium-signaling cascades common to chondrogenesis. Here we document the effects of specified PEMF parameters over mesenchymal stem cells (MSC) chondrogenic differentiation. MSCs undergoing chondrogenesis are preferentially responsive to an electromagnetic efficacy window defined by field amplitude, duration and frequency of exposure. Contrary to conventional practice of administering prolonged and repetitive exposures to PEMFs, optimal chondrogenic outcome is achieved in response to brief (10 minutes), low intensity (2 mT) exposure to 6 ms bursts of magnetic pulses, at 15 Hz, administered only once at the onset of chondrogenic induction. By contrast, repeated exposures diminished chondrogenic outcome and could be attributed to calcium entry after the initial induction. Transient receptor potential (TRP) channels appear to mediate these aspects of PEMF stimulation, serving as a conduit for extracellular calcium. Preventing calcium entry during the repeated PEMF exposure with the co-administration of EGTA or TRP channel antagonists precluded the inhibition of differentiation. This study highlights the intricacies of calcium homeostasis during early chondrogenesis and the constraints that are placed on PEMF-based therapeutic strategies aimed at promoting MSC chondrogenesis. The demonstrated efficacy of our optimized PEMF regimens has clear clinical implications for future regenerative strategies for cartilage.

Indexed as

Electromagnetic FieldsCalciumCalcium SignalingCell DifferentiationCells, CulturedChondrogenesisDose-Response Relationship, RadiationExtracellular MatrixHumansMesenchymal Stem CellsCalcium

Identifiers

PMID28842627
PMCPMC5572790
OpenAlexW2748536759

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.