ArticleMolecular plant2018
Two CYP82D Enzymes Function as Flavone Hydroxylases in the Biosynthesis of Root-Specific 4'-Deoxyflavones in Scutellaria baicalensis.
Article in Molecular plant, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed, 1 synthesis or guideline pooled it, 169 citations in OpenAlex.
- Evidence and possible mechanism ofAnnals of medicine · 2023Pooled it
- A novel C5-Horticulture research · 2026Article
- Discovery of an isoflavone biosynthetic pathway in Iridaceae plants.Nature communications · 2026Article
- Identification of an isoflavone 6-hydroxylase involved in tectorigenin biosynthesis in kudzu (Plant biotechnology (Tokyo, Japan) · 2026Article
- P450 Fusion Protein Expressed inMolecules (Basel, Switzerland) · 2026Article
- Genetic diversity and chemotypic variation of Gentiana rhodantha: insights into the genetic basis of active compound accumulation.BMC plant biology · 2026Article
- Genome-wide characterization of cytochrome P450 genes in Solanum tuberosum and StCYP481 role in salt tolerance.BMC plant biology · 2026Article
- Comparative genome analysis provides a foundation for defining salvinorin A biosynthesis in Salvia divinorum.Nature communications · 2026Article
- Dual regulation of antiviral IFN response byJournal of pharmaceutical analysis · 2026Article
- Plastid 2-oxoglutarate-dependent dioxygenases mediate stereoselective C14β-hydroxylation in cardenolide biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Genome-wide characterization of the AP2/ERF gene family inFrontiers in plant science · 2026Article
- CYP82G1 Enzyme Functions as Chromone Hydroxylase in the Biosynthesis of 6-Hydroxy-2-(2-Phenylethyl)chromone in Aquilaria sinensis.Plant biotechnology journal · 2025Article
- Sbmyb111 act as a transcriptional activator of flavonoid synthesis in Scutellaria baicalensis.Plant molecular biology · 2025Article
- CYP98A monooxygenases: a key enzyme family in plant phenolic compound biosynthesis.Horticulture research · 2025Article
- Engineering scutellarin biosynthesis in Artemisia annua.Plant cell reports · 2025Article
- GHCYP706A7 governs anthocyanin biosynthesis to mitigate ROS under alkali stress in cotton.Plant cell reports · 2025Article
- Functional evolution and diversification of CYP82D subfamily members have shaped flavonoid diversification in the genus Scutellaria.Plant communications · 2025Article
- Independent evolution of plant natural products: Formation of benzoxazinoids in Consolida orientalis (Ranunculaceae).The Journal of biological chemistry · 2025Article
- In Vitro Inducted TetraploidPlants (Basel, Switzerland) · 2024Article
- Recent advances inHeliyon · 2024Review
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
Abstract
Baicalein, wogonin, and their glycosides are major bioactive compounds found in the medicinal plant Scutellaria baicalensis Georgi. These flavones can induce apoptosis in a variety of cancer cell lines but have no effect on normal cells. Furthermore, they have many additional benefits for human health, such as anti-oxidant, antiviral, and liver-protective properties. Here, we report the isolation and characterization of two CYP450 enzymes, SbCYP82D1.1 and SbCYP82D2, which function as the flavone 6-hydroxylase (F6H) and flavone 8-hydroxylase (F8H), respectively, in S. baicalensis. SbCYP82D1.1 has broad substrate specificity for flavones such as chrysin and apigenin and is responsible for biosynthesis of baicalein and scutellarein in roots and aerial parts of S. baicalensis, respectively. When the expression of SbCYP82D1.1 is knocked down, baicalin and baicalein levels are reduced significantly while chrysin glycosides accumulate in hairy roots. SbCYP82D2 is an F8H with high substrate specificity, accepting only chrysin as its substrate to produce norwogonin, although minor 6-hydroxylation activity can also be detected. Phylogenetic analysis suggested that SbCYP82D2 might have evolved from SbCYP82D1.1 via gene duplication followed by neofunctionalization, whereby the ancestral F6H activity is partially retained in the derived SbCYP82D2.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.