Evidence map›Paper›PMID 28842193›Full record

ArticleJournal of neuroscience methods2017

Flow characterization and patch clamp dose responses using jet microfluidics in a tubeless microfluidic device.

Pedro J Resto, Abhishek Bhat, Eric Stava, Chong Lor, Elliot Merriam, Ruben E Diaz-Rivera, Robert Pearce, Robert Blick, Justin C Williams

Abstract read
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In one paragraph

Article in Journal of neuroscience methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Pedro J RestoDepartment of Mechanical Engineering, University of Puerto Rico, University of Puerto Rico at Mayagüez, Mayagüez, P.R. 00680, USA; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. Electronic address: pedroj.resto@upr.edu.
Abhishek BhatDepartment of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA.
Eric StavaDepartment of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA.
Chong LorDepartment of Anesthesiology, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Elliot MerriamDepartment of Anatomy, University of Wisconsin-Madison, Madison, WI 53706, USA.
Ruben E Diaz-RiveraDepartment of Mechanical Engineering, University of Puerto Rico, University of Puerto Rico at Mayagüez, Mayagüez, P.R. 00680, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Robert PearceDepartment of Anesthesiology, University of Wisconsin-Madison, Madison, WI, 53706, USA.
Robert BlickDepartment of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA; Institutes of Nanostructure and Solid State Physics (INF), Jungiusstrasse 11C, 20355 Hamburg, Germany; Center for Hybrid Nanostructures, Falkenried 88, 20251 Hamburg, Germany.
Justin C WilliamsDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA.

Funding

Clinical Neuroengineering Training ProgramT32EB011434 · NIBIB · UNIVERSITY OF WISCONSIN-MADISON · PI MEYERAND, MARY E., PEARCE, ROBERT A · 2011 to 2015
$869k
NIBIB NIH HHS T32 EB011434
6 · The paper itself

Abstract

backgroundSurface tension passive pumping is a way to actuate flow without the need for pumps, tubing or valves by using the pressure inside small drop to move liquid via a microfluidic channel. These types of tubeless devices have typically been used in cell biology. Herein we present the use of tubeless devices as a fluid exchange platform for patch clamp electrophysiology. NEW

methodInertia from high-speed droplets and jets is used to create flow and perform on-the-fly mixing of solutions. These are then flowed over GABA transfected HEK cells under patch in order to perform a dose response analysis.

resultsTIRF imaging and electrical recordings are used to study the fluid exchange properties of the microfluidic device, resulting in 0-90% fluid exchange times of hundreds of milliseconds. COMSOL is used to model flow and fluid exchange within the device. Patch-clamping experiments show the ability to use high-speed passive pumping and its derivatives for studying peak dose responses, but not for studying ion channel kinetics. COMPARISON WITH EXISTING METHOD(S): Our system results in fluid exchange times slower than when using a standard 12-barrel application system and is not as stable as traditional methods, but it offers a new platform with added functionality.

conclusionsSurface tension passive pumping and tubeless devices can be used in a limited fashion for electrophysiology. Users may obtain peak dose responses but the system, in its current form, is not capable of fluid exchange fast enough to study the kinetics of most ion channels.

Indexed as

Lab-On-A-Chip DevicesComputer SimulationDose-Response Relationship, DrugEquipment DesignFinite Element AnalysisGABA Agentsgamma-Aminobutyric AcidHEK293 CellsHumansKineticsMicrofluidicsMicroscopy, FluorescenceModels, BiologicalPatch-Clamp TechniquesReceptors, GABA-ASurface TensionGABA Agentsgamma-Aminobutyric AcidReceptors, GABA-AMicrofluidicsPassive pumpingPatch clampingTubeless devices

Identifiers

PMID28842193
PMCPMC8611316

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.