Evidence map›Paper›PMID 28839228›Full record

ArticleScientific reports2017

Magnetic-Immuno-Loop-Mediated Isothermal Amplification Based on DNA Encapsulating Liposome for the Ultrasensitive Detection of P-glycoprotein.

Hongmei Cao, Xueen Fang, Peng Liu, Hua Li, Weiwei Chen, Baohong Liu, Jilie Kong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Hongmei CaoDepartment of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, P.R. China.
Xueen FangDepartment of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, P.R. China. fxech@fudan.edu.cn.
Peng LiuDepartment of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, 168 Changhai road, Shanghai, 200433, China.
Hua LiDepartment of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, P.R. China.
Weiwei ChenDepartment of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, P.R. China.
Baohong LiuDepartment of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, P.R. China.
Jilie KongDepartment of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, P.R. China. jlkong@fudan.edu.cn.
Fudan University · CNChanghai Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Determination of proteins, especially low-abundance proteins with high sensitivity and specificity, is essential for characterizing proteomes and studying their biochemical functions. Herein, a novel Magnetic-Immuno-Loop-Mediated Isothermal Amplification (Im-LAMP) based on DNA-encapsulating liposomes (liposome-Im- LAMP), was developed for trace amounts of proteins. To the best of our knowledge, this is our first report about the magnetic Im-LAMP approach based on liposomes encapsulated template DNA as the detection reagent. The DNA template was released from liposomes and then initiated an Im-LAMP reaction, generating the fluorescence signal with high sensitivity and rapidity. This technique was applied for the determination of P-glycoprotein as a model protein. It was demonstrated that the technique exhibited a dynamic response to P-glycoprotein ranging from 1.6*10

Indexed as

Immunologic TechniquesMagneticsATP Binding Cassette Transporter, Subfamily B, Member 1FluorescenceLiposomesNucleic Acid Amplification TechniquesProtein BindingSensitivity and SpecificityATP Binding Cassette Transporter, Subfamily B, Member 1Liposomes

Identifiers

PMID28839228
PMCPMC5571029
OpenAlexW2749824249

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.