ArticleScientific reports2017
A systematic analysis of the RNA-targeting potential of secreted bacterial effector proteins.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- A searchable atlas of pathogen-sensitive lncRNA networks in human macrophages.Nature communications · 2025Article
- Advantages and limitations of UV cross-linking analysis of protein-RNA interactomes in microbes.Molecular microbiology · 2023Review
- Signal-noise metrics for RNA binding protein identification reveal broad spectrum protein-RNA interaction frequencies and dynamics.Nature communications · 2023Article
- The Phytophthora nucleolar effector Pi23226 targets host ribosome biogenesis to induce necrotrophic cell death.Plant communications · 2023Article
- RNA interactome capture in Escherichia coli globally identifies RNA-binding proteins.Nucleic acids research · 2023Article
- Single-cell RNA sequencing uncovers the nuclear decoy lincRNA PIRAT as a regulator of systemic monocyte immunity during COVID-19.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Development of an RNA-protein crosslinker to capture protein interactions with diverse RNA structures in cells.RNA (New York, N.Y.) · 2022Article
- Global discovery of bacterial RNA-binding proteins by RNase-sensitive gradient profiles reports a new FinO domain protein.RNA (New York, N.Y.) · 2020Article
- Noncoding RNAProceedings of the National Academy of Sciences of the United States of America · 2020Article
- An RNA-Binding Protein Secreted by a Bacterial Pathogen Modulates RIG-I Signaling.Cell host & microbe · 2019Article
- TriPepSVM: de novo prediction of RNA-binding proteins based on short amino acid motifs.Nucleic acids research · 2019Article
- Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.Nature communications · 2019Article
- RNA-binding proteins in bacteria.Nature reviews. Microbiology · 2018Review
- Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis-specific proteins.PloS one · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many pathogenic bacteria utilize specialized secretion systems to deliver proteins called effectors into eukaryotic cells for manipulation of host pathways. The vast majority of known effector targets are host proteins, whereas a potential targeting of host nucleic acids remains little explored. There is only one family of effectors known to target DNA directly, and effectors binding host RNA are unknown. Here, we take a two-pronged approach to search for RNA-binding effectors, combining biocomputational prediction of RNA-binding domains (RBDs) in a newly assembled comprehensive dataset of bacterial secreted proteins, and experimental screening for RNA binding in mammalian cells. Only a small subset of effectors were predicted to carry an RBD, indicating that if RNA targeting was common, it would likely involve new types of RBDs. Our experimental evaluation of effectors with predicted RBDs further argues for a general paucity of RNA binding activities amongst bacterial effectors. We obtained evidence that PipB2 and Lpg2844, effector proteins of Salmonella and Legionella species, respectively, may harbor novel biochemical activities. Our study presenting the first systematic evaluation of the RNA-targeting potential of bacterial effectors offers a basis for discussion of whether or not host RNA is a prominent target of secreted bacterial proteins.
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