ArticleScientific reports2017
Light-patterning of synthetic tissues with single droplet resolution.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 80 citations in OpenAlex.
- Prototissues: Assembly strategies, collective behaviors, and emerging applications.Bioactive materials · 2026Review
- ON-OFF nanopores for optical control of transmembrane ionic communication.Nature nanotechnology · 2025Article
- Narrative Review and Guide: State of the Art and Emerging Opportunities of Bioprinting in Tissue Regeneration and Medical Instrumentation.Bioengineering (Basel, Switzerland) · 2025Review
- Superstructural ordering in self-sorting coacervate-based protocell networks.Nature chemistry · 2024Article
- Sequence-independent, site-specific incorporation of chemical modifications to generate light-activated plasmids.Chemical science · 2023Article
- Engineering cellular communication between light-activated synthetic cells and bacteria.Nature chemical biology · 2023Article
- Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many.ACS synthetic biology · 2023Review
- Precise, Orthogonal Remote-Control of Cell-Free Systems Using Photocaged Nucleic Acids.Journal of the American Chemical Society · 2023Article
- Light-Activated Assembly of Connexon Nanopores in Synthetic Cells.Journal of the American Chemical Society · 2023Article
- Signal processing and generation of bioactive nitric oxide in a model prototissue.Nature communications · 2022Article
- High-throughput production of functional prototissues capable of producing NO for vasodilation.Nature communications · 2022Article
- Synthetic cell-based materials extract positional information from morphogen gradients.Science advances · 2022Article
- Review
- Droplet Microfluidics for Tumor Drug-Related Studies and Programmable Artificial Cells.Global challenges (Hoboken, NJ) · 2021Review
- A Floating Mold Technique for the Programmed Assembly of Protocells into Protocellular Materials Capable of Non-Equilibrium Biochemical Sensing.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Interfacing Living and Synthetic Cells as an Emerging Frontier in Synthetic Biology.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2021Review
- Interfacing Living and Synthetic Cells as an Emerging Frontier in Synthetic Biology.Angewandte Chemie (International ed. in English) · 2021Review
- Controlling Synthetic Cell-Cell Communication.Frontiers in molecular biosciences · 2021Review
- Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells.Scientific reports · 2020Article
- Controlling gene expression with light: a multidisciplinary endeavour.Biochemical Society transactions · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic tissues can be generated by forming networks of aqueous droplets in lipid-containing oil. Each droplet contains a cell-free expression system and is connected to its neighbor through a lipid bilayer. In the present work, we have demonstrated precise external control of such networks by activating protein expression within single droplets, by using light-activated DNA to encode either a fluorescent or a pore-forming protein. By controlling the extent of activation, synthetic tissues were generated with graded levels of protein expression in patterns of single droplets. Further, we have demonstrated reversible activation within individual compartments in synthetic tissues by turning a fluorescent protein on-and-off. This is the first example of the high-resolution patterning of droplet networks, following their formation. Single-droplet control will be essential to power subsets of compartments within synthetic tissues or to stimulate subsets of cells when synthetic tissues are interfaced with living tissues.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.