ArticleScientific reports2017
CUBIC pathology: three-dimensional imaging for pathological diagnosis.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed, 146 citations in OpenAlex.
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- Wheat Peptides Promote Recovery of the Enteric Nervous System via mAChR Activation to Relieve Constipation in Mice.ACS omega · 2026Article
- Hybrid supervised deep learning for lung adenocarcinoma diagnosis to optimize surgical strategies.NPJ precision oncology · 2026Article
- activeDISCO enables large-scale exploration of human brain anatomy by light sheet microscopy.Communications biology · 2026Article
- Rapid and Solvent-Free, 2-hydroxyethyl Methacrylate (HEMA)-Acrylamide (AAm) Copolymer-Based Optical Clearing of Tissue for Fluorescent Imaging.Bio-protocol · 2025Article
- Quantitative cytoarchitectural phenotyping of deparaffinized human brain tissues.Communications biology · 2025Article
- Decoding the human tongue of light chain amyloidosis in three dimensions (3D): three-dimensional pathological visualization drives early and precise screening of suspected cases.BMC oral health · 2025Article
- An open-source photobleacher for fluorescence imaging of large pigment-rich tissues.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Investigating hepatic fibrosis heterogeneity by three-dimensional imaging in metabolic dysfunction-associated steatotic liver disease.Scientific reports · 2025Article
- Unlocking the potential of large-scale 3D imaging with tissue clearing techniques.Microscopy (Oxford, England) · 2025Review
- A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplification.Communications biology · 2025Article
- Horizontal Imaging of Skin Tumors Using Advanced Fluorescence Techniques for Detecting Individual Malignant Cells.Acta histochemica et cytochemica · 2025Article
- Application of HEMA-AAm copolymer to achieve faster optical tissue transparency for 2D/3D fluorescence imaging.Scientific reports · 2025Article
- A multiwell plate approach to increase the sample throughput during tissue clearing.Nature protocols · 2025Review
- From morphology to single-cell molecules: high-resolution 3D histology in biomedicine.Molecular cancer · 2025Review
- 3D visualization of human colon tissue using a modified CUBIC-based tissue-clearing technique.Journal of biological methods · 2025Article
33 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
17 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The examination of hematoxylin and eosin (H&E)-stained tissues on glass slides by conventional light microscopy is the foundation for histopathological diagnosis. However, this conventional method has some limitations in x-y axes due to its relatively narrow range of observation area and in z-axis due to its two-dimensionality. In this study, we applied a CUBIC pipeline, which is the most powerful tissue-clearing and three-dimensional (3D)-imaging technique, to clinical pathology. CUBIC was applicable to 3D imaging of both normal and abnormal patient-derived, human lung and lymph node tissues. Notably, the combination of deparaffinization and CUBIC enabled 3D imaging of specimens derived from paraffin-embedded tissue blocks, allowing quantitative evaluation of nuclear and structural atypia of an archival malignant lymphoma tissue. Furthermore, to examine whether CUBIC can be applied to practical use in pathological diagnosis, we performed a histopathological screening of a lymph node metastasis based on CUBIC, which successfully improved the sensitivity in detecting minor metastatic carcinoma nodules in lymph nodes. Collectively, our results indicate that CUBIC significantly contributes to retrospective and prospective clinicopathological diagnosis, which might lead to the establishment of a novel field of medical science based on 3D histopathology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.