ArticleScientific reports2017
Lossless Three-Dimensional Parallelization in Digitally Scanned Light-Sheet Fluorescence Microscopy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array.Laser & photonics reviews · 2022Article
- Fast, multiplane line-scan confocal microscopy using axially distributed slits.Biomedical optics express · 2021Article
- Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array.Light, science & applications · 2020Article
- Fast objective coupled planar illumination microscopy.Nature communications · 2019Article
- Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited].Optics express · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
We introduce a concept that enables parallelized three-dimensional imaging throughout large volumes with isotropic 300-350 nm resolution. By staggering high aspect ratio illumination beams laterally and axially within the depth of focus of a digitally scanned light-sheet fluorescence microscope (LSFM), multiple image planes can be simultaneously imaged with minimal cross-talk and light loss. We present a first demonstration of this concept for parallelized imaging by synthesizing two light-sheets with nonlinear Bessel beams and perform volumetric imaging of fluorescent beads and invasive breast cancer cells. This work demonstrates that in principle any digitally scanned LSFM can be parallelized in a lossless manner, enabling drastically faster volumetric image acquisition rates for a given sample brightness and detector technology.
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Registered trials
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