ArticlePloS one2017
An infrared optical pacing system for screening cardiac electrophysiology in human cardiomyocytes.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Fluorescence-lifetime optical electrophysiology in contracting cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Microfluidic platforms for monitoring cardiomyocyte electromechanical activity.Microsystems & nanoengineering · 2025Review
- CommonCirculation. Genomic and precision medicine · 2024Article
- Massive field-of-view sub-cellular traction force videography enabled by Single-Pixel Optical Tracers (SPOT).Biosensors & bioelectronics · 2024Article
- Nongenetic Optical Modulation of Pluripotent Stem Cells Derived Cardiomyocytes Function in the Red Spectral Range.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Electrophysiological analysis of hyperkalemic cardiomyocytes using a multielectrode array system.Biophysics and physicobiology · 2024Article
- Emerging trends in the development of flexible optrode arrays for electrophysiology.APL bioengineering · 2023Review
- A fully-automated low-cost cardiac monolayer optical mapping robot.Frontiers in cardiovascular medicine · 2023Article
- Adipogenic Signaling Promotes Arrhythmia Substrates before Structural Abnormalities in TMEM43 ARVC.Journal of personalized medicine · 2022Article
- Elucidating arrhythmogenic right ventricular cardiomyopathy with stem cells.Birth defects research · 2022Review
- Bidirectional modulation of evoked synaptic transmission by pulsed infrared light.Scientific reports · 2022Article
- Visualizing the lipid dynamics role in infrared neural stimulation using stimulated Raman scattering.Biophysical journal · 2022Article
- Ventricular arrhythmias in mouse models of diabetic kidney disease.Scientific reports · 2021Article
- A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates.Scientific reports · 2020Article
- Human Cardiac Mesenchymal Stem Cells Remodel in Disease and Can Regulate Arrhythmia Substrates.Circulation. Arrhythmia and electrophysiology · 2020Article
- Near-infrared light driven tissue-penetrating cardiac optogenetics via upconversion nanoparticles in vivo.Biomedical optics express · 2020Article
- Identifying the Role of Block Length in Neural Heat Block to Reduce Temperatures During Infrared Neural Inhibition.Lasers in surgery and medicine · 2020Article
- Spontaneous action potentials in retinal horizontal cells of goldfish (Journal of neurophysiology · 2019Article
- Optical Investigation of Action Potential and Calcium Handling Maturation of hiPSC-Cardiomyocytes on Biomimetic Substrates.International journal of molecular sciences · 2019Article
- New Molecular Scaffolds for Fluorescent Voltage Indicators.ACS chemical biology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Human cardiac myocytes derived from pluripotent stem cells (hCM) have invigorated interest in genetic disease mechanisms and cardiac safety testing; however, the technology to fully assess electrophysiological function in an assay that is amenable to high throughput screening has lagged. We describe a fully contactless system using optical pacing with an infrared (IR) laser and multi-site high fidelity fluorescence imaging to assess multiple electrophysiological parameters from hCM monolayers in a standard 96-well plate. Simultaneous multi-site action potentials (FluoVolt) or Ca2+ transients (Fluo4-AM) were measured, from which high resolution maps of conduction velocity and action potential duration (APD) were obtained in a single well. Energy thresholds for optical pacing were determined for cell plating density, laser spot size, pulse width, and wavelength and found to be within ranges reported previously for reliable pacing. Action potentials measured using FluoVolt and a microelectrode exhibited the same morphology and rate of depolarization. Importantly, we show that this can be achieved accurately with minimal damage to hCM due to optical pacing or fluorescence excitation. Finally, using this assay we demonstrate that hCM exhibit reproducible changes in repolarization and impulse conduction velocity for Flecainide and Quinidine, two well described reference compounds. In conclusion, we demonstrate a high fidelity electrophysiological screening assay that incorporates optical pacing with IR light to control beating rate of hCM monolayers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.