ArticlePloS one2017
Single-cell profiling of dynamic cytokine secretion and the phenotype of immune cells.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Nested Nanowell Arrays for High-Throughput Quantitative Analysis of Cytokines from Single Macrophages.ACS nano medicine · 2026Article
- A few cells make the difference in monocyte interleukin release.Nature immunology · 2025Article
- Mechanisms and clinical advancements of cell-based immunotherapies in non-small cell lung cancer: an integrated perspective.Frontiers in immunology · 2025Review
- Research advances on TIL therapy for colorectal cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2024Review
- Identification of a clinically efficacious CAR T cell subset in diffuse large B cell lymphoma by dynamic multidimensional single-cell profiling.Nature cancer · 2024Article
- An immune-competent human gut microphysiological system enables inflammation-modulation by Faecalibacterium prausnitzii.NPJ biofilms and microbiomes · 2024Article
- Probing T-cell activation in nanoliter tumor co-cultures using membrane displacement trap arrays.Integrative biology : quantitative biosciences from nano to macro · 2024Article
- High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays.Nature biomedical engineering · 2023Article
- Spatially Defined Cell-Secreted Protein Detection Using Granular Hydrogels: μGeLISA.ACS biomaterials science & engineering · 2023Article
- Multiplexed analysis of signalling proteins at the single-immune cell level.Lab on a chip · 2023Article
- Who Knew? Dopamine Transporter Activity Is Critical in Innate and Adaptive Immune Responses.Cells · 2023Article
- A Computational Model of Cytokine Release Syndrome during CAR T-cell Therapy.Advanced therapeutics · 2022Article
- High-throughput single-cell antibody secretion quantification and enrichment using droplet microfluidics-based FRET assay.iScience · 2022Article
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles.Cancers · 2021Article
- A continuous flow cell culture system for precision cell stimulation and time-resolved profiling of cell secretion.Analytical biochemistry · 2021Article
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Designed improvement to T-cell immunotherapy by multidimensional single cell profiling.Journal for immunotherapy of cancer · 2021Article
- Article
- Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip.Cell reports · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Natural killer (NK) cells are a highly heterogeneous population of innate lymphocytes that constitute our first line of defense against several types of tumors and microbial infections. Understanding the heterogeneity of these lymphocytes requires the ability to integrate their underlying phenotype with dynamic functional behaviors. We have developed and validated a single-cell methodology that integrates cellular phenotyping and dynamic cytokine secretion based on nanowell arrays and bead-based molecular biosensors. We demonstrate the robust passivation of the polydimethylsiloxane (PDMS)-based nanowells arrays with polyethylene glycol (PEG) and validated our assay by comparison to enzyme-linked immunospot (ELISPOT) assays. We used numerical simulations to optimize the molecular density of antibodies on the surface of the beads as a function of the capture efficiency of cytokines within an open-well system. Analysis of hundreds of individual human peripheral blood NK cells profiled ex vivo revealed that CD56dimCD16+ NK cells are immediate secretors of interferon gamma (IFN-γ) upon activation by phorbol 12-myristate 13-acetate (PMA) and ionomycin (< 3 h), and that there was no evidence of cooperation between NK cells leading to either synergistic activation or faster IFN-γ secretion. Furthermore, we observed that both the amount and rate of IFN-γ secretion from individual NK cells were donor-dependent. Collectively, these results establish our methodology as an investigational tool for combining phenotyping and real-time protein secretion of individual cells in a high-throughput manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.