Evidence map›Paper›PMID 28836378›Full record

ArticleCNS neuroscience & therapeutics2017

Ethyl pyruvate does not require microglia for mediating neuroprotection after excitotoxic injury.

Philipp Pieroh, Daniel-Christoph Wagner, Chalid Ghadban, Gerd Birkenmeier, Faramarz Dehghani

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in CNS neuroscience & therapeutics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Philipp PierohDepartment of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
Daniel-Christoph WagnerFraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany.
Chalid GhadbanDepartment of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
Gerd BirkenmeierInstitute of Biochemistry, University of Leipzig, Leipzig, Germany.
Faramarz DehghaniDepartment of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.ORCID http://orcid.org/0000-0002-7615-8854
Martin Luther University Halle-Wittenberg · DEFraunhofer Institute for Cell Therapy and Immunology · DELeipzig University of Applied Sciences · DEUniversity Hospital Leipzig · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsEthyl pyruvate (EP) mediates protective effects after neuronal injury. Besides a direct conservation of damaged neurons, the modulation of indigenous glial cells has been suggested as one important mechanism for EP-related neuroprotection. However, the specific contribution of glial cells is still unknown.

methodsOrganotypic hippocampal slice cultures (OHSC) were excitotoxically lesioned by 50 μmol/L N-methyl-D-aspartate (NMDA, for 4 hours) or left untreated. In an additional OHSC subset, microglia was depleted using the bisphosphonate clodronate (100 μg/mL) before lesion. After removal of NMDA, EP containing culture medium (0.84 μmol/L, 8.4 μmol/L, 42 μmol/L, 84 μmol/L, 168 μmol/L) was added and incubated for 72 hours. OHSC were stained with propidium iodide to visualize degenerating neurons and isolectin IB

resultsEP significantly reduced neurodegeneration following induced excitotoxicity except for 168 μmol/L. For 84 μmol/L, a reduction in the microglia cells was observed. Microglia depletion did not affect neuronal survival after EP treatment. EP decelerated astrocytic wound closure at 48 hours after injury.

conclusionEP-mediated neuroprotection seems to be mediated by astrocytes and/or neurons.

Indexed as

AnimalsAstrocytesCicatrixHippocampusMicrogliaNeuronsNeuroprotective AgentsN-MethylaspartatePyruvatesRats, Sprague-DawleyTissue Culture Techniquesethyl pyruvateNeuroprotective AgentsN-MethylaspartatePyruvatesethyl pyruvateexcitotoxicityorganotypic hippocampal slice culture

Identifiers

PMID28836378
PMCPMC6492706
OpenAlexW2749318047

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.